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Human Inflammation Array C3

Human Inflammation Array C3

Item no.	AAH-INF-3-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Carcinoma Glioma Hematology Leukemia Myeloma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Mesothelioma Arrays By Organ Lung Cancer Colorectal Cancer Testicular & Prostate Cancer Uterine, Ovarian & Cervical Cancer Antibody Arrays
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Ebihara N, Yamagami S, Yokoo S, Amano S, Murakami A. Involvement of C-C Chemokine Ligand 2-CCR2 Interaction in Monocyte-Lineage Cell Recruitment of Normal Human Corneal Stroma. J Immunol. 2007,178:3288-3292.<br />2. Vesey DA, Suen JY, Seow V, et al. PAR2-induced inflammatory responses in human kidney tubular epithelial cells. Am J Physiol Renal Physiol. 2013 Mar 15,304(6):F737-F750.<br />3. PAR2-induced inflammatory responses in human kidney tubular epithelial cells<br />4. Lin PW, Simon PO, Gewirtz AT, Neish AS, et al. Paneth Cell Cryptdins Act in Vitro as Apical Paracrine Regulators of the Innate Inflammatory Response. J Biol Chem. 2004,279(19):19902-19907.<br />5. Ide T, Hirase T, Hazuku A,Ikeda Y, Node K. Angiotensin II Increases Expression of IP-10 and the Renin-Angiotensin System in Endothelial Cells. Hypertens Res. 2008,31:1257–1267. doi:10.1291/hypres.31.1257 <br />6. Said N, Najwer I, Socha M,Fulton D,Mok S,et al.SPARC Inhibits LPA-Mediated Mesothelial–Ovarian Cancer Cell Crosstalk.Neoplasia.2007,9:23–35<br />7. Long D, Blake S, Song X, Lark M, Loeser R. Human articular chondrocytes produce IL-7 and respond to IL-7 with increased production of matrix metalloproteinase-13. Arthritis Res Ther. 2008,10:R23 doi:10.1186/ar2376<br />8. Miyoshi N, Oubrahim H, Chock PB, Stadtman ER. Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis. PNAS. 2006,103(6):1727–1731.<br />9. Fukuda EY, Lad SP, Mikolon DP, Iacobelli-Martinez M, Li E. Activation of Lipid Metabolism Contributes to Interleukin-8 Production during Chlamydia trachomatis Infection of Cervical Epithelial Cells. Infect Immunity. 2005,73(7):4017-4024.<br />10. Trinidad AG, de la Puerta ML, Fernandez N, Bayon Y, Crespo MS, Alonso A. Coupling of C3bi to IgG inhibits the tyrosine phosphorylation signaling cascade downstream Syk and reduces cytokine induction in monocytes. J Leukoc.Biol. 2006,79:1073-1082.<br />11. Shiraki Y, Ishibashi Y, Hiruma M, Nishikawa A, Ikeda S. Cytokine secretion profiles of human keratinocytes during Thrichophyton tonsurans and Arthroderma benhamiae infections. J Med Microbiol 2006, 55: 1175-1185.<br />12. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011,186:453–463.<br />13. Said NA, Najwer I, Socha MJ, Fulton DJ, Mok SC, Motamed K. SPARC Inhibits LPA-Mediated Mesothelial-Ovarian Cancer Cell Crosstalk. Neoplasia. 2007, 9(1):23–35.<br />14. Cebalo L, Markotic A. Chemokine Production Predominates in Human Monocytes Infected with Tula Virus. Viral Immunol. 2007,20:206–213.<br />15. Matsunaga K,Yanagisawa S,Ichikawa T, Akamatsu K, Koarai A, et al. Molecular Mechanism of the Additive Effects of Leukotriene Modifier in Asthmatic Patients Receiving Steroid Therapy. Allergol Int. 2009,58:89–96.<br />16. Matsunaga K, Ichikawa T, Yanagisawa S, Akamatsu K, Koarai A, et al.Clinical Application of Exhaled Breath Condensate Analysis in Asthma: Prediction of FEV 1 Improvement by Steroid Therapy. Respiration. 2009,78:393–398.<br />17. Ichikawa T,Matsunaga K,Minakata Y, Yanagisawa S, Ueshima K ,et al.Possible Impact of Salivary Infl uence on Cytokine Analysis in Exhaled Breath Condensate. Anal Chem Insights. 2007:2 85–92<br />18. Shoji J, Inada N, Sawa M. Antibody Array-Generated Cytokine Profile of Tears of Patients with Vernal Keratoconjunctivitis or Giant Papillary Conjunctivitis. Jpn J Ophthalmol. 2006,50:195-204.<br />19. Rowley A, Baker S, Shulman S, Garcia F, Guzman-Cottrill J, Detection of Antigen in Bronchial Epithelium and Macrophages in Acute Kawasaki Disease by Use of Synthetic Antibody. J Infect Dis. 2004,190:856-865.<br />20. Fierro NA, Pedraza-Alva G, Rosenstein Y. TCR-Dependent Cell Response is Modulated by the Timing of CD43 Engagement. The Journal of Immunology June 15, 2006 vol. 176 no. 12 7346-7353 <br />21. Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]<br />22. He Y., Liu S., Leone S., Newburg DS. Human colostrum oligosaccharides modulate major immunologic pathways of immature human intestine. Mucosal Immunology, 2014, Published online, April 2. doi: 10.1038/mi.2014.20<br />23. Pyrc K, Bijl A , Dijkman R, Jebbink M, Snoek M, et al. HCoV-NL63 induces interleukin-6 and interleukin-8 expression. In Dissertation by Pyrc KA. Virus discovery and human coronavirus NL63. University of Amsterdam. 2007,121–132.<br />24. Sugitharini V., et al. TLR-mediated inflammatory response to neonatal pathogens and co-infection in neonatal immune cells. Cytokine 69 (2014) 211–217. http://dx.doi.org/10.1016/j.cyto.2014.06.003<br />25. Guerra-Laso J., et al. Microarray analysis of Mycobacterium tuberculosis-infected monocytes reveals IL-26 as a new candidate gene for tuberculosis susceptibility. Immunology, August 2014 epub ahead of print. DOI: 10.1111/imm.12371<br />26. Shi L., et al. Hypothermia Stimulates Glioma Stem Spheres to Spontaneously Dedifferentiate Adjacent Non-stem Glioma Cells. Cellular and Molecular Neurobiology Sept 2014, epub ahead of print. DOI 10.1007/s10571-014-0114-1<br />27. Jemima, E. Functional characterization of histamine H4 receptor on human mast cells. Molecular Immunology, Volume 62, Issue 1, Pages 19-28. NOV 2014
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, IL-13, IL-4, IL-8, IL-6, G-CSF, IL-17, IL-2, IL-16, RANTES, M-CSF, MIP-1 alpha, GM-CSF, IL-6 sR, IL-7, IL-11, IL-15, MCP-1, ICAM-1, IL-3, MIG, TIMP-2, IP-10, PDGF-BB, Eotaxin-2, MCP-2, MIP-1 beta, IL-12 p70, I-309, MIP-1 delta, Eotaxin-1, TGF beta 1, IL-12 p40, TNF beta, TNF alpha, sTNFRI, sTNFRII
Available
Number of Targets Detected	40
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	CCL1 \| CCL11 \| CCL15 \| CCL2 \| CCL24 \| CCL3 \| CCL4 \| CCL5 \| CCL8 \| CSF1\| CSF2 \| CSF3 \| CXCL10 \| CXCL8 \| CXCL9 \| ICAM1 \| IFNG \| IL10 \| IL11 \| IL12A \| IL12B \| IL13 \| IL15 \| IL16 \| IL17A \| IL1A \| IL1B \| IL2 \| IL3 \| IL4 \| IL6 \| IL6R \| IL7 \| LTA \| PDGFB \| TGFB1 \| TIMP2 \| TNF \| TNFRSF1A \| TNFRSF1B
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Human Inflammation Antibody Array C3 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P??s Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.