Item no. |
AAH-HSP-1-8 |
Manufacturer |
Raybiotech
|
Amount |
8 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Human |
Host |
Hamster - Armenian |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
HSP90, HSP40, HSP70, HSP60, Ubiquitin, HSP27, GRP75, HSP10, HSP32 |
Available |
|
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Detection Method |
Chemiluminescence |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Kit Components |
- Human HSP Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Number of Targets Detected |
9 |
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Result Output |
Semi-Quantitative |
Short description |
RayBio C-Series Human Heat Shock Protein Antibody Array Kit. Detects 9 Human heat shock proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates. |
Solid Support |
Membrane |
Storage |
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes a -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
GRP75, HSP10, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, Ubiquitin+1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.