Item no. |
AAH-GF-1-8 |
Manufacturer |
Raybiotech
|
Amount |
8 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Human (Homo sapiens) |
Host |
Hamster - Armenian |
Citations |
1. Tanaka H., et al. Liver Condition Affects Bovine Oocyte Qualities by Changing the Characteristics of Follicular Fluid and Plasma. Reprod Domest Anim. 2013 Aug,48(4):619-26. doi: 10.1111/rda.12135 2. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression 3. Christov C, Cretien F, Abou-Kalil R, Bassez G, Vallet G, et al. Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 2007,18:1397–1409. 4. De S, Razorenova O, McCabe NP, O'Toole T, Qin J, Byzova TV. VEGF-integrin interplay controls tumor growth and vascularization. Proc Natl Acad Sci USA. 2005,102(21):7589–7594. 5. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008,8:e18. 6. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008, 102: 77-85 7. Okuda H., Kobayashi A., Xia B., Watabe M., et al. Hyaluronan Synthase HAS2 Promotes Tumor Progression in Bone by Stimulating the Interaction of Breast Cancer Stem–Like Cells with Macrophages and Stromal Cells. Cancer Research. January 15, 2012 72, 537 8. Al-aidaroos A., et al. Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 2013,123(8):3459–3471. doi:10.1172/JCI66824. 9. Dilwali, S et al. Primary culture of human Schwann and schwannoma cells: Improved and simplified protocol. Hearing Research 2014. Epub Ahead of Print DOI: 10.1016/j.heares.2014.05.006 10. Glinskii OV, Huxley VH, Glinskii VV, Rubin LJ, Glinsky VV (2013) Pulsed Estrogen Therapy Prevents Post-OVX Porcine Dura Mater Microvascular Network Weakening via a PDGF-BB-Dependent Mechanism. PLoS ONE 8(12): e82900. doi:10.1371/journal.pone.0082900 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
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Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
Number of Targets Detected |
41 |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Chemiluminescence |
Quantitative/Semi-Quantitative |
Semi-Quantitative |
Solid Support |
Membrane |
Gene Symbols |
AREG | CSF1 | CSF1R | CSF2 | CSF3 | EGF | EGFR | FGF2 | FGF4 | FGF6|Â FGF7 | FIGF | FLT4 | GDNF | HBEGF | HGF | IGF1 | IGF1R | IGF2 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IGFBP6 | KDR | KIT | KITLG | NGF | NTF3 | NTF4 | PDGFA | PDGFB | PDGFRA | PDGFRB | PGF | TGFA | TGFB1 | TGFB2 | TGFB3 | VEGFA |
Kit Components |
Human Growth Factor Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual |
Other Materials Required |
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline |
Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis |
Product Features |
Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.