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Human Cytokine Array G5

Human Cytokine Array G5

Item no.	AAH-CYT-G5-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Senescence Arrays By Organ Lung Cancer Skin Cancer Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Testicular & Prostate Cancer Gastric Cancer Brain Cancer Hepatic Cancer Lymphatic System Cancer Bone Cancer Antibody Arrays
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Rath BH, Fair JM, Jamal M, Camphausen K, Tofilon PJ (2013) Astrocytes Enhance the Invasion Potential of Glioblastoma Stem-Like Cells. PLoS ONE 8(1): e54752. doi:10.1371/journal.pone.0054752<br />2. Zhen A., Krutzik S., Levin B., et al. CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection. Journal of Virology published online ahead of print on 18 June 2014. doi:10.1128/JVI.00616-14<br />3. Hayashi T, Hideshima T, Nguyen A N, Munoz O,Podar K,et al.Transforming Growth Factor Receptor I Kinase Inhibitor Down-Regulates Cytokine Secretion and Multiple Myeloma Cell Growth in the Bone Marrow icroenvironment.Clin Cancer Res. 2004 10, 7540 <br />4. Boucharaba A, Serre C-M, Guglielmi J, Bordet J-C, Clezardin P, Peyruchaud O. The type 1 lysophosphatidic acid receptor is a target for therapy in bone metastases. Proc Natl Acad Sci USA. 2006,103(25):9643-9648.<br />5. Li J, Sidell N. Growth-related oncogene produced in human breast cancer cells and regulated by Syk protein-tyrosine kinase. Int J Cancer. 2005,117:14–20.<br />6. Huang RP, Li S, Yang W,Chen L, Yao C, et al. Early Growth Response-1 Suppresses Human Fibrosarcoma Cell Invasion and Angiogenesis.Cancer Genom Proteomics.2006,3: 71-82 <br />7. Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009,139(7):1315–1326.<br />8. De Ceuninck F, Dassencourt L, Anract P. The inflammatory side of human chondrocytes unveiled by antibody microarrays. Biochem Biophys Res Comm. 2004,323:960–969.<br />9. Leea Y,Chioub T,Tzengd W,Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008,249:116–122 <br />10. Pislaru SV, Harbuzariu A, Gulati R, Witt T, et al. Magnetically Targeted Endothelial Cell Localization in Stented Vessels. J Am Coll Cardiol. 2006, 48(9):1839-1845.<br />11. Wong CK, Cheung PFY, Ip WK, Lam CWK. Interleukin-25–Induced Chemokines and Interleukin-6 Release from Eosinophils Is Mediated by p38 Mitogen-Activated Protein Kinase, c-Jun N-Terminal Kinase, and Nuclear Factor-kB. Am J Respir Cell Mol Biol. 2005,33:186–194.<br />12. Wong CK, Cheung PFY, Lam CWK. Leptin-mediated cytokine release and migration of eosinophils: Implications for immunopathophysiology of allergic inflammation. Eur J Immunol. 2007,37:2337–2348.<br />13. Acosta JC, O'Loghlen A, Banito A, Guijarro MV, Augert A, et al. Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence. Cell. 2008,133:1006–1018.<br />14. Yokoyama T, Komori A, Nakamura M, Takii Y, et al. Human intrahepatic bilary epithelial cells function in innate immunity by producing IL-6 and IL-8 via the TLR4-NF-kB and -MAPK signaling pathways. Liver Int. 2006,26: 467-476.<br />15. Grossmann C, Podgrabinska S, Skobe M, Ganem D. Activaction of NF-kB by the Latent vFLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus is Required for the Spindle Shape of Virus-Infected Endothelial Cells and Contributes to Their Proinflammatory Phenotype. J Virol. 2006: 80(14):7179-7185.<br />16. Sivashanmugam P, Tang L, Daaka Y. Interleukin 6 Mediates the Lysophosphatidic Acid-regulated Cross-talk between Stromal and Epithelial Prostate Cancer Cells. J Biol Chem. 2004,279(20):21154-21150.<br />17. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006,27:1086-1095.<br />18. Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R. Gene and protein characteristics reflect functional diversity of CD56-dim and CD56-bright NK cells. J Leuk Biol. 2006,80:1529-1541.<br />19. Rhee SH, Keates AC, Moyer MP, Pothoulakis C. MEK Is a Key Modulator for TLR5-induced Interleukin-8 and MIP2alpha Gene Expression in Non-transformed Human Colonic Epithelial Cells. J Biol Chem. 2004, 279(24):25179-25188.<br />20. Boniotto M, Jordan WJ, Eskdale J, Tossi A, Antcheva N, et al. Human beta-Defensin 2 Induces a Vigorous Cytokine Response in Peripheral Blood Mononuclear Cells. Antimicrob Agents Chemother. 2006,50(4):1433-1441.<br />21. Chandra J, McCormick TS, Imamura Y, Mukherjee PK, Ghannoum MA.Interaction of Candida albicans with Adherent Human Peripheral Blood Mononuclear Cells Increases C. albicans Biofilm Formation and Results in Differential Expression of Pro- and Anti-Inflammatory Cytokines. Infect Immun. 2007,75(5):2612–2620.<br />22. Kastenbauer S, Angele B, Sporer B, Pfister H-W, Koedel U. Patterns of protein expression in infectious meningitis: A cerebrospinal fluid protein array analysis. J Neuroimmunol. 2005,16:134-139.<br />23. Krishnan C, Kaplin AI, Graber JS, Darman JS, Kerr DA. Recurrent transverse myelitis following neurobrucellosis: Immunologic features and beneficial response to immunosuppression. J Neurovirol. 2005,11:225-231.<br />24. Rupprecht TA, Pfister HW, Angele B, Kastenbauer S, Wilske B, Koedel U. The chemokine CXCL13 (BLC): A putative diagnostic marker for neuroborreliosis. Neurology. 2005, 65:448-450.<br />25. Kverka M, Burianova J, Lodinova-Zadnikova R, Kocuirkova I, et al. Cytokine Profiling in Human Colostrum and Milk by Protein Array. Clin Chem. 2007, 53(5):955-962.<br />26. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006,27:1086-1095.<br />27. Celis JE, Gromova I, Moreira J M, Cabezon T,et al. Impact of proteomics on bladder cancer research. Pharmacogenomics. 2004,5(4):381-394.<br />28. Hashimoto I, Wada J, Hida A, Baba M. Elevated Serum Monoctye Chemoattractant Protein-4 and Chronic Inflammation in Overweight Subjects. Obesity. 2006:14:799-811.<br />29. Hon K E, Ching G, Wong K, Leung T, Leung A. A Pilot Study to Explore the Usefulness of Antibody Array in Childhood Atopic Dermatitis.JOURNAL OF THE NATIONAL MEDICAL ASSOCIATION. 2008,500-504<br />30. Leea Y,Chioub T,Tzengd W,Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008,249:116-122 <br />31. Sharma M, Arnason JT, Burt A, Hudson JB. Echinacea Extracts Modulate the Pattern of Chemokine and Cytokine Secretion in Rhinovirus-Infected and Uninfected Epithelial Cells. Phytother Res. 2006, 20:147-152.<br />32. Chung Y-L, Jian JJ-M, Cheng SH, Tsai SYC, Chuang VP, et al. Sublethal Irradiation Induces Vascular Endothelial Growth Factor and Promotes Growth of Hepatoma Cells: Implications for Radiotherapy of Hepatocellular Carcinoma. Clin Cancer Res. 2006,12(9):2706-2715.<br />33. Weiser C, Stumpf D, Grillhosl C, Lengenfelder D, et al. Regulated and constitutive expression of anti-inflammatory cytokines by nontransforming herpesvirus saimiri vectors. Gene Ther. 2005,12:395-406.
ECLASS 5.1	32161000
ECLASS 6.1	32161000
ECLASS 8.0	32161000
ECLASS 9.0	32161000
ECLASS 10.0.1	32161000
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
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Available
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Detection Method	Fluorescence Laser Scanner
Features	High sensitivity and specificity Low sample volume (10-100 ul per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
Kit Components	Human Cytokine Antibody Array G5 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor Lysis Buffer Adhesive Plastic Strips 30 ml Centrifuge Tube Manual
Number of Targets Detected	80
Other Materials Required	Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Block array surface Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Result Output	Semi-Quantitative
Short description	RayBio G-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.
Solid Support	Glass Slide
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	Angiogenin, BDNF, BLC, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20, CCL22, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CK beta 8-1, CX3CL1, CXCL1, CXCL10, CXCL12 alpha, CXCL13, CXCL6, CXCL7, CXCL8, CXCL9, EGF, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GM-CSF, GRO, GRO alpha, HGF, I-309, IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-16, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, KGF, Leptin, LIF, LIGHT, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIF, MIG, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MPIF-2, NAP-2, NT-3, NT-4, Oncostatin M, Osteopontin, Osteoprotegerin, PARC, PDGF-BB, PlGF, PPBP, RANTES, SCF, SDF-1 alpha, SPP1, TARC, TCA-3, TCK-1, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNFRSF11B, TNFSF14, TNFSF1B, TPO, VEGF-A

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