Comparison

Human Cytokine Array C8

Item no. AAH-CYT-8-8
Manufacturer Raybiotech
Amount 8 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Category
Type Array
Specific against Human (Homo sapiens)
Host Hamster - Armenian
Citations 1. Yang CC, Shih YH, Ko MH, Hsu SY,Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton’s Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008,3(10):e3336. doi:10.1371/journal.pone.0003336
2. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis
3. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013,73:662–671.
4. Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007, 67(8):3646–3653.
5. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007,2(9): e941. doi:10.1371/journal.pone.0000941
6. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M,et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008,93: 2255–2262
7. Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006,26(5):483-490.
8. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008,8:e18.
9. Le Bras G., et al. TGFbeta loss activates ADAMTS-1-mediated EGF-dependent invasion in a model of esophageal cell invasion. Experimental Cell Research Available online 23 July 2014. DOI: 10.1016/j.yexcr.2014.07.021
10. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Published Online First August 4, 2014, doi: 10.1158/1541-7786.MCR-14-0159
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
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Shipping condition Cool pack
Available
Manufacturer - Type
Arrays|Cytokine Arrays|Antibody Arrays|Membrane Antibody Arrays
Manufacturer - Targets
AcPL, Activin A, ALCAM, B7-1, BMP-5, BMP-7, c-kit, Cardiotrophin-1, CCL23, CD102, CD117, CD122, CD14, CD170, CD31, CD62L, CD80, CDH5, Common gamma Chain, CT-1, CXCL10, CXCL12 beta, CXCL16, DR6, E-Selectin, Endoglin, ErbB3, Fas Ligand, ICAM-2, IGF-2, IL-1 R2, IL-10 R beta, IL-13 R alpha 2, IL-18 BP alpha, IL-18 R beta, IL-2 R beta, IL-2 R gamma, IL-21 R, IL-5 R alpha, IL-9, IP-10, L-Selectin, LAP/TGF beta 1, Leptin R, LIF, M-CSF R, MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1, NGFR, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PECAM-1, Prolactin, SCF R, SDF-1 beta, Siglec-5, TGF alpha, TGF beta 2, Tie-1, Tie-2, TIMP-4, TNFRSF16, TNFRSF21, TNFSF6, VE-Cadherin, VEGFR2, VEGFR3
Storage Conditions
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Description
RayBio C-Series Human Cytokine Antibody Array 8 Kit. Detects 54 Human Cytokines. Suitable for all liquid sample types.
Number of Targets Detected
54
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Solid Support
Membrane
Kit Components
Human Cytokine Antibody Array C8 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Product Features
Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex
Result Output
Semi-Quantitative

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 8 Sample Kit
Available: In stock
available

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