Comparison

Human Cytokine Array C5

Item no. AAH-CYT-5-8
Manufacturer Raybiotech
Amount 8 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Category
Type Array
Specific against Human
Host Hamster - Armenian
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Meyer-Siegler KL, Leifheit EC, Vera PL. Inhibition of macrophage migration inhibitory factor decreases proliferation and cytokine expression in bladder cancer cells. BMC Cancer. 2004,4:34.<br />7. Boucharaba A, Serre C-M, Guglielmi J, Bordet J-C, Clezardin P, Peyruchaud O. The type 1 lysophosphatidic acid receptor is a target for therapy in bone metastases. Proc Natl Acad Sci USA. 2006,103(25):9643-9648.<br />8. Li J, Sidell N. Growth-related oncogene produced in human breast cancer cells and regulated by Syk protein-tyrosine kinase. Int J Cancer. 2005,117:14–20.<br />9. Huang RP, Li S, Yang W,Chen L, Yao C, et al. Early Growth Response-1 Suppresses Human Fibrosarcoma Cell Invasion and Angiogenesis.Cancer Genom Proteomics.2006,3: 71-82 <br />10. Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009,139(7):1315–1326.<br />11. De Ceuninck F, Dassencourt L, Anract P. The inflammatory side of human chondrocytes unveiled by antibody microarrays. Biochem Biophys Res Comm. 2004,323:960–969.<br />12. Leea Y,Chioub T,Tzengd W,Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008,249:116–122 <br />13. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1690<br />14. Pislaru SV, Harbuzariu A, Gulati R, Witt T, et al. Magnetically Targeted Endothelial Cell Localization in Stented Vessels. J Am Coll Cardiol. 2006, 48(9):1839-1845.<br />15. Wong CK, Cheung PFY, Ip WK, Lam CWK. Interleukin-25–Induced Chemokines and Interleukin-6 Release from Eosinophils Is Mediated by p38 Mitogen-Activated Protein Kinase, c-Jun N-Terminal Kinase, and Nuclear Factor-kB. Am J Respir Cell Mol Biol. 2005,33:186–194.<br />16. Wong CK, Cheung PFY, Lam CWK. Leptin-mediated cytokine release and migration of eosinophils: Implications for immunopathophysiology of allergic inflammation. Eur J Immunol. 2007,37:2337–2348.<br />17. Acosta JC, O'Loghlen A, Banito A, Guijarro MV, Augert A, et al. Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence. Cell. 2008,133:1006–1018.<br />18. Yokoyama T, Komori A, Nakamura M, Takii Y, et al. Human intrahepatic bilary epithelial cells function in innate immunity by producing IL-6 and IL-8 via the TLR4-NF-kB and -MAPK signaling pathways. Liver Int. 2006,26: 467-476.<br />19. Deroanne CF, Hamelryckx D, Ho TTG, Lambert CA, Catroux P, et al. Cdc42 downregulates MMP-1 expression by inhibiting the ERK1/2 pathway. J Cell Sci. 2005,118:1173-1183.<br />20. Grossmann C, Podgrabinska S, Skobe M, Ganem D. Activaction of NF-kB by the Latent vFLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus is Required for the Spindle Shape of Virus-Infected Endothelial Cells and Contributes to Their Proinflammatory Phenotype. J Virol. 2006: 80(14):7179-7185.<br />21. Sivashanmugam P, Tang L, Daaka Y. Interleukin 6 Mediates the Lysophosphatidic Acid-regulated Cross-talk between Stromal and Epithelial Prostate Cancer Cells. J Biol Chem. 2004,279(20):21154-21150.<br />22. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006,27:1086-1095.<br />23. Huang L, Zhao A, Wong F, Ayala JM, et al. Leukotriene B4 Strongly Increases Monocyte Chemoattractant Protien-1 in Human Monocytes. Arterioscler Thromb Vasc Biol. 2004,24:1783-1788.<br />24. Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R. Gene and protein characteristics reflect functional diversity of CD56-dim and CD56-bright NK cells. J Leuk Biol. 2006,80:1529-1541.<br />25. Rhee SH, Keates AC, Moyer MP, Pothoulakis C. MEK Is a Key Modulator for TLR5-induced Interleukin-8 and MIP2alpha Gene Expression in Non-transformed Human Colonic Epithelial Cells. J Biol Chem. 2004, 279(24):25179-25188.<br />26. Yin L. Chondroitin Synthase 1 Is a Key Molecule in Myeloma Cell-Osteoclast Interactions. J Biol Chem. 2005,280(16):15666-15672.<br />27. Boniotto M, Jordan WJ, Eskdale J, Tossi A, Antcheva N, et al. Human beta-Defensin 2 Induces a Vigorous Cytokine Response in Peripheral Blood Mononuclear Cells. Antimicrob Agents Chemother. 2006,50(4):1433-1441.<br />28. Chandra J, McCormick TS, Imamura Y, Mukherjee PK, Ghannoum MA.Interaction of Candida albicans with Adherent Human Peripheral Blood Mononuclear Cells Increases C. albicans Biofilm Formation and Results in Differential Expression of Pro- and Anti-Inflammatory Cytokines. Infect Immun. 2007,75(5):2612–2620.<br />29. Bogatkevich GS, Ludwicka-Bradley A, Highland KB, Hant F, Nietert PJ, et al. Impairment of the Antifibrotic Effect of Hepatocyte Growth Factor in Lung Fibroblasts from African Americans. Arthritis Rheum. 2007,56(7):2432–2442.<br />30. Kastenbauer S, Angele B, Sporer B, Pfister H-W, Koedel U. Patterns of protein expression in infectious meningitis: A cerebrospinal fluid protein array analysis. J Neuroimmunol. 2005,16:134-139.<br />31. Krishnan C, Kaplin AI, Graber JS, Darman JS, Kerr DA. Recurrent transverse myelitis following neurobrucellosis: Immunologic features and beneficial response to immunosuppression. J Neurovirol. 2005,11:225-231.<br />32. Rupprecht TA, Pfister HW, Angele B, Kastenbauer S, Wilske B, Koedel U. The chemokine CXCL13 (BLC): A putative diagnostic marker for neuroborreliosis. Neurology. 2005, 65:448-450.<br />33. Kverka M, Burianova J, Lodinova-Zadnikova R, Kocuirkova I, et al. Cytokine Profiling in Human Colostrum and Milk by Protein Array. Clin Chem. 2007, 53(5):955-962.<br />34. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006,27:1086-1095.<br />35. Walt DR, Blicharz TM, Hayman RB, Rissin DM, et al. Microsensor Arrays for Saliva Diagnostics. Ann NY Acad Sci. 2007,1098:389–400.<br />36. Sack RA, Conradi L, Krumholz D, Beaton A, Sathe S, Morris C. Membrane Array Characterization of 80 Chemokines, Cytokines and Growth Factors in Open- and Closed-eye Tears: Angiogenin and Other Defense System Constituents. Invest Ophtahalmol Vis Sci. 2005,45:1228-1238.<br />37. Celis JE, Gromova I, Moreira J M, Cabezon T,et al. Impact of proteomics on bladder cancer research. Pharmacogenomics. 2004,5(4):381-394.<br />38. Hashimoto I, Wada J, Hida A, Baba M. Elevated Serum Monoctye Chemoattractant Protein-4 and Chronic Inflammation in Overweight Subjects. Obesity. 2006:14:799-811.<br />39. Celis J E, Gromov P, Cabezo T, Moreira JM Ambartsumian N, et al.Proteomic Characterization of the Interstitial Fluid Perfusing the Breast Tumor Microenvironment. Mol Cell Proteomics. 2004,3:327–344. <br />40. Rovin B, Song H, Hebert L, Nadasdy T, Nadasdy G, et al. Plasma, urine, and renal expression of adiponectin in human systemic lupus erythematosus. Kidney Int. 2005,68:1825–1833<br />41. He T, Smith LA, Harrington S, Nath KA, Caplice NM, Katusic ZS. Transplantation of Circulating Endothelial Progenitor Cells Restores Endothelial Function of Denuded Rabbit Carotid Arteries. Stroke. 2004,35,2378–2384,<br />42. Hon K E, Ching G, Wong K, Leung T, Leung A. A Pilot Study to Explore the Usefulness of Antibody Array in Childhood Atopic Dermatitis.JOURNAL OF THE NATIONAL MEDICAL ASSOCIATION. 2008,500-504<br />43. Leea Y,Chioub T,Tzengd W,Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008,249:116-122 <br />44. Chung Y-L, Jian JJ-M, Cheng SH, Tsai SYC, Chuang VP, et al. Sublethal Irradiation Induces Vascular Endothelial Growth Factor and Promotes Growth of Hepatoma Cells: Implications for Radiotherapy of Hepatocellular Carcinoma. Clin Cancer Res. 2006,12(9):2706-2715.<br />45. Chung Y-L, Jian JJ-M, Cheng SH, Tsai SYC, Chuang VP, et al. Sublethal Irradiation Induces Vascular Endothelial Growth Factor and Promotes Growth of Hepatoma Cells: Implications for Radiotherapy of Hepatocellular Carcinoma. Clin Cancer Res. 2006,12(9):2706-2715.<br />46. Lehmann MH, Masanetz S, Kramer S, Erfle V. HIV-1 Nef upregulates CCL1/MCP-1 expression in astrocytes in a myristolation- and calmodulin-dependent manner. J Cell Sci. 2006, 119:4520-4530.<br />47. Sharma M, Arnason JT, Burt A, Hudson JB. Echinacea Extracts Modulate the Pattern of Chemokine and Cytokine Secretion in Rhinovirus-Infected and Uninfected Epithelial Cells. Phytother Res. 2006, 20:147-152.<br />48. Chung Y-L, Jian JJ-M, Cheng SH, Tsai SYC, Chuang VP, et al. Sublethal Irradiation Induces Vascular Endothelial Growth Factor and Promotes Growth of Hepatoma Cells: Implications for Radiotherapy of Hepatocellular Carcinoma. Clin Cancer Res. 2006,12(9):2706-2715.<br />49. Weiser C, Stumpf D, Grillhosl C, Lengenfelder D, et al. Regulated and constitutive expression of anti-inflammatory cytokines by nontransforming herpesvirus saimiri vectors. Gene Ther. 2005,12:395-406.<br />50. Zhou Q, Desta T, Fenton M, Graves DT, Amar S. Cytokine Profiling of Macrophages Exposed to Porphyromonas gingivalis, Its Lipopolysaccharide, or Its FimA Protein. Infect Immun. 2005,73(2):935–943.<br />51. Reverter J, Nadal J, Fernandez-Novell J, Ballester J, Ramio-Lluch L, et al. Tyrosine Phosphorylation of Vitreous Inflammatory and Angiogenic Peptides and Proteins in Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 2009,50:1378–1382. <br />52. Shir, A et al. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. PLoS Med. Volume 3, Issue 1, Pages e6, Jan 2006<br />53. Hayashi, T et al. Transforming growth factor beta receptor I kinase inhibitor down-regulates cytokine secretion and multiple myeloma cell growth in the bone marrow microenvironment. Transforming growth factor beta receptor I kinase inhibitor down-regulates cytokine secretion and multiple myeloma cell growth in the bone marrow microenvironment. Transforming growth factor beta receptor I kinase inhibitor down-regulates cytokine secretion and multiple myeloma cell growth in the bone marrow microenvironment. Clin. Cancer Res. Volume 10, Issue 22, Pages 7540-6, Nov 2004<br />54. Okuda H., Kobayashi A., Xia B., Watabe M., et al. Hyaluronan Synthase HAS2 Promotes Tumor Progression in Bone by Stimulating the Interaction of Breast Cancer Stem–Like Cells with Macrophages and Stromal Cells. Cancer Research. January 15, 2012 72, 537 <br />55. McDowell K., Begley L., Mor-Vaknin N., Markovitz D., Macoska J. Leukocytic Promotion of Prostate Cellular Proliferation. TheProstate 70:377-389 (2010)<br />56. Sun, X., Mao, Y. et al. IL-6 secreted by cancer-associated fibroblasts induces tamoxifen resistance in luminal breast cancer. Oncogene 2014. Epub Ahead of Print. DOI: 10.1038/onc.2014.158<br />57. Kim JK., et al. Tumoral RANKL activates astrocytes that promote glioma cell invasion through cytokine signaling. Cancer Letters. Received: May 12, 2014, Accepted: July 14, 2014, Published Online: July 28, 2014 DOI: http://dx.doi.org/10.1016/j.canlet.2014.07.034<br />58. Hendrayani SF., et al. The Cytokine IL-6 Reactivates Breast Stromal Fibroblasts through Transcription Factor STAT3-dependent Up-regulation of the RNA Binding Protein AUF1.<br />59. Fernandez-Pisonero I., et al. Ferna´ ndez-Pisonero I, Lo´pez J, Onecha E, Duen˜ as AI, Maeso P, et al. (2014) Synergy between Sphingosine 1-Phosphate and Lipopolysaccharide Signaling Promotes an Inflammatory, Angiogenic and Osteogenic Response in Human Aortic Valve Interstitial Cells. PLoS ONE 9(10): e109081. doi:10.1371/journal.pone. 0109081<br />60. Chen L., et al. Evaluation of immune inhibitory cytokine profiles in epithelial ovarian carcinoma. J. Obstet. Gynaecol. Res. Vol. 35, No. 2: 212–218, April 2009<br />61. Shimabukuro-Vornhagen A., et al. Inhibition of Protein Geranylgeranylation Specifically Interferes with CD40-Dependent B Cell Activation, Resulting in a Reduced Capacity To Induce T Cell Immunity. The Journal of Immunology, October 2014 epub ahead of print. doi:10.4049/jimmunol.1203436
ECLASS 5.1 32161000
ECLASS 6.1 32161000
ECLASS 8.0 32161000
ECLASS 9.0 32161000
ECLASS 10.0.1 32161000
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
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Available
Number of Targets Detected
80
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Gene Symbols
ANG | BDNF | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18 | CCL2 | CCL20| CCL22 | CCL23 | CCL24 | CCL26 | CCL4 | CCL5 | CCL7 | CCL8 | CSF1 | CSF2 | CSF3 | CX3CL1 | CXCL1 | CXCL10 | CXCL12 | CXCL13 | CXCL2 | CXCL3 | CXCL5 | CXCL6 | CXCL8 | CXCL9 | EGF | FGF4 | FGF6 | FGF7 | FGF9 | FLT3LG | GDNF | HGF | IFNG | IGF1 | IGFBP1 | IGFBP2 | IGFBP3 | IGFBP4 | IL10 | IL12A | IL13 | IL15 | IL16 | IL1A | IL1B | IL2 | IL3 | IL4 | IL5 | IL6 | IL7 | KITLG | LEP | LIF | LTA | MIF | NTF3 | NTF4 | OSM | PDGFB | PGF | PPBP | SPP1 | TGFB1 | TGFB2 | TGFB3 | THPO | TIMP1 | TIMP2 | TNF | TNFRSF11B | TNFSF14 | TPO | VEGFA
Application Information
Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker/ Key Factor Screening
Identifying Key Factors
Confirming a Biological Process
Kit Components
Human Cytokine Antibody Array C5 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-
Conjugated H
R
P
Detection Buffer C
Detection Buffer D
Lysis Buffer8-
Well Incubation Tray
Plastic Sheets
Array Templates
Manual
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as U
V
P's Chemi
Doc-
It® or Epi
Chem I
I Benchtop Darkroom or G
E's Image
Quant™ L
A
S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with H
R
P-
Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Product Features
Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 8 Sample Kit
Available: In stock
available

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