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Human Cytokine Array C3

Human Cytokine Array C3

Item no.	AAH-CYT-3-8
Manufacturer	Raybiotech
Amount	8 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling By Cell Type Carcinoma Hematology Leukemia Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Testicular & Prostate Cancer Lymphatic System Cancer Uterine, Ovarian & Cervical Cancer Antibody Arrays
Type	Array
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Citations	1. Role of the IL-6-JAK1-STAT3-Oct-4 pathway in the conversion of non-stem cancer cells into cancer stem-like cells 2. Tadros Y, Ruiz-Deya G, Crawford BE, Thomas R, Ardel-Mageed AR. In Vivo Proteomic Analysis of Cytokine Expression in Laser Capture-Microdissected Urothelial Cells of Obstructed Ureteropelvic Junction Procured by Laparoscopic Dismembered Pyeloplasty. J Endourol. 2003,17(5):333-336. 3. Abrahams VM, Bole-Aldo P, Kim YM, Straszewski-Chavez SL, et al. Divergent Trophoblast Responses to Bacterial Products Mediated by TLRs. J Immunol. 2005,173: 4286–4296. 4. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007,43:1117–1124. 5. Wang S, Yang J, Qian J, Wezeman M, Kwak LW, Yi Q. Tumor evasion of the immune system: inhibiting p38 MAPK signaling restores the function of dendritic cells in multiple myeloma. Blood. 2006,107:2431-2439. 6. Wang F-X, Xu Y, Sullivan J, Souder E, et al. IL-7 is a potent and proviral strain–specific inducer of latent HIV-1 cellular reservoirs of infected individuals on virally suppressive HAART. J Clin Invest. 2005,155:128–137. 7. Sconocchia G, Keyvanfar K, El Ouriaghli F, Grube M, et al. Phenotype and function of a CD56+ peripheral blood monocyte. Leukemia. 2005,19:69–76 8. Eldeen M B,Deshmane S L,Simbiri K, Khalili K, Amini S,et al.MH2 domain of Smad3 reduces HIV-1 Tat-induction of cytokine secretion. J Neuroimmunol. 2006,176:174 – 180 9. Gerrits J H,Wetering J,Weimar W,Besouw N M. Donor-Reactive Cytokine Production After HLA-Identical Living Related Kidney Transplantation: A Protein-Array Analysis.Transplantation Proceedings,2006,38, 2825–2827 10. Rethi B, Gogolak P, Szatmari I, Veres A, et al. SLAM/SLAM interactions inhibit CD40-induced production of inflammatory cytokines in monocyte-derived dendritic cells. Blood. 2006,107:2821-2829. 11. Li J,Bentsman G,Potash M,Volsky D.Human immunodeficiency virus type 1 efficiently binds to human fetal astrocytes and induces neuroinflammatory responses independent of infection.BMC Neuroscience.2007, 8:31 12. Fiorucci M,Boulant S, Fournillier A, Abraham J, Lavergne J,et al.Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in hepatic injuries.J Gen Virol. 2007,88:1149–1162 13. Potian JA, Aviv H, Ponzio NM, Harrison JS, Rameshwar P. Veto-Like Activity of Mesenchymal Stem Cells: Functional Discrimination Between Cellular Responses to Alloantigens and Recall Antigens. J Immunol. 2003,17(1)3426.3434. 14. Julia P, Daniel S.Effect of gonococcal lipooligosaccharide variation on human monocytic cytokine profile.BMC Microbiology. 2007,7:7 15. Brown JM, Boysen MS, Chung S, Fabiy O, et al. Conjugated Linoleic Acid Induces Human Adipocyte Delipidation: Autocrine/Paracrine Signaling by Adipokines. J Biol Chem. 2004,279(25):26735-26747. 16. Xu Y Kulkoshy J, Pomerantz RJ. HIV-1-mediated apoptosis of neuronal cells: Proximal molecular mechanisms of HIV-1-induced encephalopathy. Proc Natl Acad Sci USA. 2004,101(18):7071-7075. 17. Pavlova-Jelinkova L, Rozkova D, Pecharova B, Bartova J, et al. Gliadin Fragments Induce Phenotypic and Functional Maturation of Human Dendritic Cells. J Immunol. 2005,175: 7038–7045. 18. Pavlova-Jelinkova L, Rozkova D, Pecharova B, Bartova J, et al. Gliadin Fragments Induce Phenotypic and Functional Maturation of Human Dendritic Cells. J Immunol. 2005,175: 7038–7045. 19. Abbott DW, Yang Y, Hutti JE, Madhavarapu S, Kelliher MA, Cantley LC. Coordinated Regulation of Toll-Like Receptor and NOD2 Signaling by K63-Linked Polyubiquitin Chains. Mol Cell Biol. 2007,27(17):6012-6025. 20. Benitez S, Camacho M, Bancells C, Vila L, Sanchez-Quesada JL, Ordonez-Llanos J. Wide proinflammatory effect of electronegative low-density lipoprotein on human endothelial cells assayed by a protein array. Biochim Biophys Acta. 2006,1761:1014-1021. 21. Ryzhov S, Goldstein AE, Matafonov A, Zeng D, Biaggioni I, Feoktistov I. Adenosine-Activated Mast Cells Induce IgE Synthesis by B Lymphocytes: An A(2B)-Mediated Process Involving Th2 Cytokines IL-4 and IL-13 with Implications for Asthma. J Immunol. 2004, 172:7726-7733. 22. Tang X, Marciano DL, Leeman SE, Amar S. LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines. PNAS. 2005,102(14):5132-5137. 23. Patrone JB, Bish SE, Stein DC. TNF-alpha-Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response. J Immunol. 2006,177:1314-1322. 24. Gogolak P, Rethi B, Szatmari I, Lanyi A, et al. Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPAR-gamma. Blood. 2007, 109:643-652. 25. DeCicco KL, Tanaka T, Andreola F, De Luca LM. The effect of thalidomide on non-small cell lung cancer (NSCLC) cell lines: possible involvement of the PPAR-gamma pathway. Carcinogenesis. 2004,25:1805-1812. 26. Chiu TT, Leung WY, Moyer MP, Strieter RM, Rozengurt E. Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-kB. Am J Physiol Cell Physiol.2007,292:C767–C777. 27. Robson RL, Reed NA, Horvat RT. Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties. BMC Infect Dis. 2006,6:71. Available at http://www.biomedcentral.com/1471-2334/6/71. Last accessed Jan 25, 2008. 28. Bogdanski D, Esenwein SA, Prymak O, Epple M, Muhr G, Koller M. Release of Leucocyte Mediators after Contact to Coated and Non-coated NITI-SMA. Biomedizinische Technik Jg. 2004,49:562-563. 29. Ritter M, Mennerich D, Weith A, Seither P. Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm. 2005, 2:16. Available at: http://www.journal-inflammation.com/content/2/1/16. Last accessed Jan 25, 2008. 30. Tay SS, McCormack A, Rose ML. Effect of Cognate Human CD4+ T Cell and Endothelial Cell Interactions Upon Chemokine Production. Transplant. 2004,78(7):987-994. 31. Duenas AI, Aceves M, Orduna A, Diaz R, Crespo MS, Garcia-Rodriguez C. Francisella tularensis LPS induces the production of cytokines in human monocytes and signals via Toll-like receptor 4 with much lower potency than E. coli LPS. Intl Immunol. 2006,18(5):785-795. 32. Marks DJB, Harbord MWN, MacAllister R, Rahman FZ, Young J, et al. Defective acute inflammation in Crohn's disease: a clinical investigation. Lancet. 2006,367:668-678. 33. Nick JA, Coldren CD, Geraci MA, Poch KR, et al. Recombinant human activated protein C reduces human endotoxin–induced pulmonary inflammation via inhibition of neutrophil chemotaxis. Blood. 2004,104(13):3878-3885. 34. Kaplin AI, Deshpande DM, Scott E, Krishnan C, Carmen JS, et al. IL-6 induces regionally selective spinal cord injury in patients with the neuroinflammatory disorder transverse myelitis. J Clin Invest. 2005,115:2731-2741. 35. Nichols MT, Gidey E, Matzakos T, Dahl R, et al. Secretion of Cytokines and Growth Factors Into Autosomal Dominant Polycystic Kidney Disease Liver Cyst Fluid. Hepatology. 2004,40:836-846. 36. Coppinger JA, O'Connor R, Wynne K, Flanagan M, Sullivan M, et al. Moderation of the platelet releasate response by aspirin. Blood. 2007,109:4786-4792. 37. Bailey L, Kuroyanagi Y, Franco-Penteado CF, Conran N, Costa FF, et al. Expression of the gamma-globulin gene is sustained by the cAMP-dependent pathway in beta-thalassaemia. Br J Haematol. 2007,138:382–395. 38. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007,43:1117–1124. 39. Patrone J, Bish S, Stein D. TNF-alpha -Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response.J Immunol. 2006,177:1314–1322. 40. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005,16:256–265. 41. Gaurnier-Hausser A, Rothman VL, Dimitrov S, Tuszynski GP. The Novel Angiogenic Inhibitor, Angiocidin, Induces Differentiation of Monocytes to Macrophages. Cancer Res July 15, 2008 68, 5905 42. Carroll TP, Greene CM, Taggart CC, Bowie AG, O'Neill SJ, McElvaney NG. Viral Inhibition of IL-1- and Neutrophil Elastase-Induced Inflammatory Responses in Bronchial Epithelial Cells. J Immunol. December 1, 2005 vol. 175 no. 11 7594-7601 43. Lee S., Kim K., Min K., Kim K., Chang S., Kim J. Angiogenin Reduces Immune Inflammation via inhibition of TANK-Binding Kinase 1 Expression in Human Corneal Fibroblast Cells. Mediators of Inflammation Volume 2014 (2014), Article ID 861435, 12 pages 44. Riverso M., Kortenkamp A., Silva E., Non-tumorigenic epithelial cells secrete MCP-1 and other cytokines that promote cell division in breast cancer cells by activating ERalpha via PI3 K/Akt/mTOR signaling. The International Journal of Biochemistry & Cell Biology 2014, June Epub Ahead of Print. http://dx.doi.org/10.1016/j.biocel.2014.05.023 45. Abrahams, VM et al. A role for TLRs in the regulation of immune cell migration by first trimester trophoblast cells. A role for TLRs in the regulation of immune cell migration by first trimester trophoblast cells. A role for TLRs in the regulation of immune cell migration by first trimester trophoblast cells. J. Immunol. Volume 175, Issue 12, Pages 8096-104, Dec 2005 46. Abrahams, VM et al. A role for TLRs in the regulation of immune cell migration by first trimester trophoblast cells. A role for TLRs in the regulation of immune cell migration by first trimester trophoblast cells. A role for TLRs in the regulation of immune cell migration by first trimester trophoblast cells. J. Immunol. Volume 175, Issue 12, Pages 8096-104, Dec 2005 47. Scheel, B et al. Toll-like receptor-dependent activation of several human blood cell types by protamine-condensed mRNA. Toll-like receptor-dependent activation of several human blood cell types by protamine-condensed mRNA. Toll-like receptor-dependent activation of several human blood cell types by protamine-condensed mRNA. Eur. J. Immunol. Volume 35, Issue 5, Pages 1557-66, May 2005 48. Godek J., et al. Angiocidin inhibits breast cancer proliferation through activation of epidermal growth factor receptor and nuclear factor kappa (NF-?B). Experimental and Molecular Pathology. Volume 90, Issue 3, June 2011, Pages 244–251 49. Li Y., et al. Myokine IL-15 regulates the crosstalk of co-cultured porcine skeletal muscle satellite cells and preadipocytes. Mol Biol Rep 2014, Date: 07 Aug 2014. DOI 10.1007/s11033-014-3646-z 50. Muccioli M., et al. Toll-Like Receptors as Novel Therapeutic Targets for Ovarian Cancer. ISRN Oncology Volume 2012 (2012), Article ID 642141, 8 pages http://dx.doi.org/10.5402/2012/642141 51. El-Ghonaimy E., et al. Positive lymph-node breast cancer patients: Activation of NF-kappaB in tumor associated leukocytes stimulates cytokines secretion that promotes metastasis via CC-chemokine receptor CCR7. The FEBS Journal, October 2014. DOI: 10.1111/febs.13124
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	EGF, IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, Leptin, SCF, IL-13, IL-4, IL-8, SDF-1, G-CSF, VEGF-A, IL-2, IGF-1, RANTES, M-CSF, MCP-3, GM-CSF, IL-5, IL-7, IL-15, MCP-1, ENA-78, IL-3, MDC, MIG, Oncostatin M, TARC, PDGF-BB, MCP-2, GRO, I-309, MIP-1 delta, TGF beta 1, TNF beta, Thrombopoietin, TNF alpha, Angiogenin, IL-12 p40/p70, GRO alpha
Shipping condition	Cool pack
Available
Number of Targets Detected	42
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Solid Support	Membrane
Kit Components	Human Cytokine Antibody Array C3 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Result Output	Semi-Quantitative
Short description	RayBio C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	Angiogenin, CCL1, CCL15, CCL17, CCL2, CCL22, CCL5, CCL7, CCL8, CXCL1, CXCL12 alpha, CXCL8, CXCL9, EGF, ENA-78, G-CSF, GM-CSF, GRO, GRO alpha, I-309, IFN-gamma, IGF-1, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, Leptin, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MDC, MIG, MIP-1 delta, Oncostatin M, PDGF-BB, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TGF beta 1, Thrombopoietin, TNF alpha, TNF beta, TNFSF1B, TPO, VEGF-A

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.