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Human Cytokine Array C2000

Human Cytokine Array C2000

Item no.	AAH-CYT-2000-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Senescence Enzymes Fatty Acid Synthesis Arrays By Organ Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Renal Cancer Bone Cancer Glial Cell Markers Neuronal Cell Markers Antibody Arrays
Type	Array
Specific against	Human
Citations	1. Yang CC, Shih YH, Ko MH, Hsu SY,Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton’s Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008,3(10):e3336. doi:10.1371/journal.pone.0003336 2. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis 3. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013,73:662–671. 4. Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007, 67(8):3646–3653. 5. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007,2(9): e941. doi:10.1371/journal.pone.0000941 6. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M,et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008,93: 2255–2262 7. Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006,26(5):483-490. 8. Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007, 81(14):7584–7597. 9. Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007, 81(14):7584–7597. 10. Shimizu, Masaki, Mondo Kuroda, and Michio Konishi. "Extensive Serum Biomarker Analysis in Patients with Enterohemorrhagic Escherichia Coli O111-induced Hemolytic-uremic Syndrome." Cytokine. ScienceDirect, Mar. 2014. Web. 11. Fujita K,Ewing C M ,Sokoll L J, Elliott D J, Cunningham M, et al.Cytokine Profiling of Prostatic Fluid From Cancerous Prostate Glands Identifies Cytokines Associated With Extent of Tumor and Inflammation. Prostate. 2008,68:872–882. 12. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis 13. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008,8:e18. 14. Wang T, Wang YY, Zhou R, et al. Bioactive proteins in healthy pregnancies and preeclampsia: relevance to hypertension and proteinuria. Chin Med J. 2013,126(11):2015–2020. 15. Wang T, Wang YY, Zhou R, et al. Bioactive proteins in healthy pregnancies and preeclampsia: relevance to hypertension and proteinuria. Chin Med J. 2013,126(11):2015–2020. 16. Belletti B, Vaidya J, Andrea S, Entschladen F, Roncadin M, et al. Targeted Intraoperative Radiotherapy Impairs the Stimulation of Breast Cancer Cell Proliferation and Invasion Caused by Surgical Wounding. Clin Cancer Res March 1, 2008 14, 1325 17. Belletti B, Vaidya J, Andrea S, Entschladen F, Roncadin M, et al. Targeted Intraoperative Radiotherapy Impairs the Stimulation of Breast Cancer Cell Proliferation and Invasion Caused by Surgical Wounding. Clin Cancer Res March 1, 2008 14, 1325 18. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Published Online First August 4, 2014, doi: 10.1158/1541-7786.MCR-14-0159 19. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 – 1883
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	EGF, Adiponectin, BDNF, bFGF, EGFR, IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, Leptin, MMP-1, MMP-3, MMP-9, Osteoprotegerin, PLGF, TIMP-1, TIMP-4, VEGFR2, BMP-7, SCF, IL-13, MMP-13, IL-4, IL-8, IL-6, SDF-1, Fractalkine, G-CSF, GDNF, HGF, MIF, LIF, IGFBP-2, IL-17, CD14, ICAM-2, CNTF, VEGF-A, IL-2, Prolactin, PARC, IL-16, BTC, IGF-1, Axl, RANTES, VE-Cadherin, Endoglin, M-CSF, MIP-1 alpha, MCP-3, GM-CSF, IL-5, IL-7, IL-11, IL-15, IL-9, MCP-1, Activin A, Amphiregulin, ENA-78, E-Selectin, GCP-2, ICAM-1, IL-3, Lymphotactin, MDC, MIG, NT-4, Oncostatin M, PECAM-1, TARC, TECK, TIMP-2, IP-10, SDF-1 beta, PDGF-AA, PDGF-BB, BMP-5, BMP-4, Eotaxin-2, Eotaxin-3, MCP-2, MCP-4, MIP-1 beta, CTACK, GRO, I-TAC, VEGF-D, PDGF-AB, IL-12 p70, HCC-4, I-309, NAP-2, FGF-9, ALCAM, VEGFR3, MIP-1 delta, Tie-1, Cardiotrophin-1, Eotaxin-1, IGFBP-1, ErbB3, TGF beta 1, FGF-6, FGF-4, TGF beta 2, TGF beta 3, BMP-6, IL-12 p40, TNF beta, Thrombopoietin, BLC, MIP-3 alpha, MIP-3 beta, TNF alpha, NT-3, IGFBP-3, Angiopoietin 2, Fas Ligand, TGF alpha, IL-21R, L-Selectin, IGF-2, Leptin R, uPAR, Tie-2, Angiogenin, ICAM-3, LAP, MPIF-1, TRAIL R4, GITR, IGFBP-4, Dtk, AgRP, TRAIL R3, PDGF R beta, PDGF R alpha, M-CSF R, Light, sTNFRI, IL-6 R, IL-5 R alpha, IL-18 BP alpha, IL-13 R alpha 2, IL-10 R beta, IL-1 ra, sTNFRII, IGFBP-6, GRO alpha, Flt-3 Ligand, FGF-7, IL-2 R gamma, SCF R, b-NGF, Siglec-5, IL-2 R beta, CK beta 8-1, NGF R, MSP-alpha chain, IL-1 RI, IL-2 Ra, sgp130, IGF-1 sR, GITR-Ligand, IL-1 R4/ST2, CCL-28, B7-1(CD80), CXCL-16, DR6 (TNFRSF21), Fas/TNFRSF6, IL-1 RII, IL-18 R beta
Available
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Kit Components	Human Cytokine Antibody Array C2000 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Number of Targets Detected	120
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Result Output	Semi-Quantitative
Short description	RayBio C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.
Solid Support	Membrane
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	AcPL, ACRP30, Activin A, Adiponectin, AgRP, ALCAM, Amphiregulin, Angiogenin, Angiopoietin-2, Apo-1, AXL, B7-1, BDNF, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-5, BMP-6, BMP-7, BTC, c-kit, Cardiotrophin-1, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD102, CD105, CD117, CD122, CD14, CD170, CD31, CD50, CD54, CD62L, CD80, CK beta 8-1, CNTF, Common gamma Chain, CT-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL16, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, DR6, Dtk, E-Selectin, EGF, EGFR, ENA-78, Endoglin, Eotaxin-1, Eotaxin-2, Eotaxin-3, ErbB1, ErbB3, Fas, Fas Ligand, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, HCC-4, HGF, I-309, I-TAC, ICAM-1, ICAM-2, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R2, IL-1 R4, IL-1 Ra, IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15, IL-16, IL-17, IL-18 BP alpha, IL-18 R beta, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-21 R, IL-3, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8, IL-9, IP-10, KGF, L-Selectin, LAP/TGF beta 1, Leptin, Leptin R, LIF, LIGHT, Lymphotactin, M-CSF, M-CSF R, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1, MPIF-2, MSP alpha/beta, NAP-2, NGFR, NT-3, NT-4, Oncostatin M, Osteoprotegerin, PARC, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, PlGF, PPBP, Prolactin, RANTES, SCF, SCF R, SDF-1 alpha, SDF-1 beta, Siglec-5, ST2, TARC, TCA-3, TECK, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10D, TNFRSF11B, TNFRSF16, TNFRSF18, TNFRSF21, TNFRSF6, TNFSF14, TNFSF18, TNFSF1B, TNFSF6, TPO, TRAIL R3, TRAIL R4, uPAR, VE-Cadherin, VEGF-A, VEGF-D, VEGFR2, VEGFR3, XCL1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AAH-CYT-2000-4.pdf

AAH-CYT-2000-4-datasheet.pdf

AAH-CYT-2000-4-safety-datasheet.pdf