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Human Cytokine Array C1000

Human Cytokine Array C1000

Item no.	AAH-CYT-1000-2
Manufacturer	Raybiotech
Amount	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Senescence Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Heart & Blood Cancer Testicular & Prostate Cancer Renal Cancer Lymphatic System Cancer Glial Cell Markers Neuronal Cell Markers Antibody Arrays
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Ishitsuka K, Hideshima T, Neri P, Vallet S,Shiraishi N,et al.p38 mitogen-activated protein kinase inhibitor LY2228820 enhances bortezomib-induced cytotoxicity and inhibits osteoclastogenesis in multiple myeloma, therapeutic implications. Br J Haematol.2008,141:598–606, 2. Ishibashi Y, Sugita T, Nishikawa A. Cytokine secretion pro¢le of human keratinocytes exposed to Malassezia yeasts.FEMS Immunol Med Microbiol.2006(48)400–409 3. Celis JE, Moreira JMA, Gromova I, Cabezon T, et al. Towards discovery-driven translational research in breast cancer. FEBS J. 2004,272:2-15. 4. Falkensammer C, Johrer K, Gander H, Ramoner R, Putz T, et al.IL-4 inhibits the TNF-a induced proliferation of renal cell carcinoma (RCC) and cooperates with TNF-a to induce apoptotic and cytokine responses by RCC: implications for antitumor immune responses. Cancer Immunol Immunother.2006, 55: 1228–1237 DOI 10.1007/s00262-006-0122-1 5. Dietze-Schroeder D, Sell H, Uhlig M, Koenen M, Eckel J. Autocrine Action of Adiponectin on Human Fat Cells Prevents the Release of Insulin Resistance-Inducing Factors. Diabetes. 2005,54:2003-2011. 6. Klimcakova E, Moro C, Mazzucotelli A,Lolmede K,Viguerie N,et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. Biochem Biophys Res Comm.2007,358(3):897–902 7. Moro C, Klimeakova E, Lolmede K, Berlan M, et al. Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia. 2007, 50:1038–1047. 8. Wang WE, Yang D, Li L, et al. PHD2 Silencing Enhances the Survival and Paracrine Function of Transplanted Adipose-Derived Stem Cells in Infarcted Myocardium. Circ. Res. 2013 May 21. 9. Wang Y, Cai D, Brendel C, Barett C, Erben P, et al. Adaptive secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates imatinib and nilotinib resistance in BCR/ABL+ progenitors via JAK-1/STAT-5 pathway activation. Blood. 2007,109:2147–2155. 10. Giroux M, Denis F. Human CD1d-unrestricted NKT cells release chemokines upon Fas engagement. Blood. 2005,105(2):703–710. 11. Hartiala P, Hytonen J, Pelkonen J, Kimppa K, et al. Transcriptional response of human dendritic cells to Borrelia garinii---defective CD38 and CCR7 expression detected. J Leuk Biol. 2007, 82:33-43. 12. The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner 13. Ancuta P, Wang J, Gabuzda D. CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interacting with CX3CL1-expressing endothelial cells. J Leuk Biol. 2006,80:1156-1164. 14. Maury E, Ehala-Aleksejev K, Guiot Y, Detry R, Vandenhooft A, Brichard SM. Adipokines oversecreted by omental adipose tissue in human obesity. Am J Physiol Endocrinol Metab. 2007, 293:E656–E665. 15. Henrichot E, Juge-Aubry CE, Pernin A, Pache J-C, et al. Production of Chemokines by Perivascular Adipose Tissue: A Role in the Pathogenesis of Atherosclerosis? Aterioscler Thromb Vasc Biol. 2005,25:2594-2599 16. Fu SY, Su GY, McKinley SH, Yen MT. Cytokine expression in pediatric subperiosteal orbital abscesses. Can J Ophthalmol. 2007,42:865-869. 17. Celis JE, Moreira JMA, Gromova I, Cabezon T, et al. Towards discovery-driven translational research in breast cancer. FEBS J. 2005,272: 18. Celis JE, Moreira JMA, Cabezon T, Gromov P, et al. Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients: Toward Dissecting The Molecular Circuitry of Epithelial-Adipocyte Stromal Cell Interactions. Mol Cell Proteomics. 2005,4:492-522. 19. Celis JE, Gromov P, Moreira JMA, Cabezon T, et al. Apocrine Cysts of the Breast: Biomarkers, Origin, Enlargement, and Relation with Cancer Phenotype. Mol Cell Proteomics. 2006,5:432-483. 20. Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, et al. Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins. Nat Med. 2007, 13(11):1359–1362. 21. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007,53:42–52. 22. Zhang X, Jin J P, Tang Y N, Speer D, Sujkowska D, et al. IFN-1a Inhibits the Secretion of Th17-Polarizing Cytokines in Human Dendritic Cells via TLR7 Up-Regulation.J. Immunol. 2009,182:3928–3936 23. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007,53:42–52. 24. Osman L, Yacoub MH, Latif N, Amrani M, Chester AH. Role of Human Valve Interstitial Cells in Valve Calcification and Their Response to Atorvastatin. Circulation. 2006,114(suppl.1):I547–I552. 25. Lisco A, Grivel JC, Biancotto A, Vanpouille C, et al. Viral Interactions in Human Lymphoid TissueL Human Herpesvirus 7 Suppresses the replication of CCF5-Tropic Human Immunodeficiency Virus Type 1 via CD4 Modulation. J Virol. 2007,81(2):708–717. 26. Klimcakova E, Moro C, Mazzucotelli A,Lolmede K,Viguerie N,et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. BBRC.2007,358(3):897-902 27. Klimcakova E, Moro C, Mazzucotelli A,Lolmede K,Viguerie N,et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. BBRC.2007,358(3):897-902 28. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005,16:256–265. 29. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008, 102: 77-85 30. Skopkova M, Penesova A, Sell H, Radikova Z, Vlcek M, et al. Protein Array Reveals Differentially Expressed Proteins in Subcutaneous Adipose Tissue in Obesity.Obesity (Silver Spring). 2007,15(10):2396–2406. 31. Giroux, M et al. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. Blood Volume 105, Issue 2, Pages 703-10, Jan 2005 32. Shih Y., Wang M., Zhou J., Peng H., Lee D., Chiu J. Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21. Gut 2014 Epub ahead of print June 17 2014, doi:10.1136/gutjnl-2013-306302 33. Boucek RJ., et al. Ex Vivo Paracrine Properties of Cardiac Tissue: Effects of Chronic Heart Failure. J of Heart and Lung Transpl. Available online 17 July 2014 DOI: 10.1016/j.healun.2014.07.010 34. Chung E., et al. CXCL5 abundant in the wound fluid at the late phase of wound healing, possibly promoting migration of mesenchymal stem cells and vascular tube formation. Tissue Engineering and Regenerative Medicine August 2014, Volume 11, Issue 4, pp 317-322 35. Lee YC., et al. BMP4 Promotes Prostate Tumor Growth in Bone through Osteogenesis. Cancer Research 71(15), 5194–203. DOI: 10.1158/0008-5472.CAN-10-4374 36. Kavitha, C. V., Deep, G., Gangar, S. C., Jain, A. K., Agarwal, C. and Agarwal, R. (2014), Silibinin inhibits prostate cancer cells- and RANKL-induced osteoclastogenesis by targeting NFATc1, NF-kappaB, and AP-1 activation in RAW264.7 cells. Mol. Carcinog., 53: 169–180. doi: 10.1002/mc.21959 37. Gromov P., et al. Tumor interstitial fluid — A treasure trove of cancer biomarkers. Biochimica et Biophysica Acta 1834 (2013) 2259–2270. http://dx.doi.org/10.1016/j.bbapap.2013.01.013 38. Lin Y., et al. A panel of four cytokines predicts the prognosis of patients with malignant gliomas. J Neurooncol (2013) 114:199–208, DOI 10.1007/s11060-013-1171-x 39. Zhang Y, Liu H, Wang L, Yang F, Hu Y, et al. (2013) Comparative Study of the Cytokine/Chemokine Response in Children with Differing Disease Severity in Enterovirus 71-Induced Hand, Foot, and Mouth Disease. PLoS ONE 8(6): e67430. doi:10.1371/journal.pone.0067430 40. Hastie A., et al. Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes . Journal of Allergy and Clinical Immunology, Volume 125, Issue 5, May 2010, Pages 1028–1036.e13. DOI:10.1016/j.jaci.2010.02.008
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
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Available
Number of Targets Detected	120
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	ADIPOQ \| AGRP \| ANG \| ANGPT2 \| AREG \| AXL \| BDNF \| BMP4 \| BMP6 \| BTC\| CCL1 \| CCL11 \| CCL13 \| CCL15 \| CCL16 \| CCL17 \| CCL18 \| CCL19 \| CCL2 \| CCL20 \| CCL22 \| CCL23 \| CCL24 \| CCL25 \| CCL26 \| CCL27 \| CCL28 \| CCL3 \| CCL4 \| CCL5 \| CCL7 \| CCL8 \| CNTF \| CSF1 \| CSF2 \| CSF3 \| CX3CL1 \| CXCL1 \| CXCL11 \| CXCL12 \| CXCL13 \| CXCL2 \| CXCL3 \| CXCL5 \| CXCL6 \| CXCL8 \| CXCL9 \| EGF \| EGFR \| FAS \| FGF2 \| FGF4 \| FGF6 \| FGF7 \| FGF9 \| FIGF \| FLT3LG \| GDNF \| HGF \| ICAM1 \| ICAM3 \| IFNG \| IGF1 \| IGF1R \| IGFBP1 \| IGFBP2 \| IGFBP3 \| IGFBP4 \| IGFBP6 \| IL10 \| IL11 \| IL12A \| IL12B \| IL13 \| IL15 \| IL16 \| IL17A \| IL1A \| IL1B \| IL1R1 \| IL1RL1 \| IL1RN \| IL2 \| IL2RA \| IL3 \| IL4 \| IL5 \| IL6 \| IL6R \| IL6ST \| IL7 \| KITLG \| LEP \| LTA \| MIF \| MST1 \| NGF \| NTF3 \| NTF4 \| OSM \| PDGFB \| PGF \| PLAUR \| PPBP \| TGFB1 \| TGFB3 \| THPO \| TIMP1 \| TIMP2 \| TNF \| TNFRSF10C \| TNFRSF10D \| TNFRSF11B \| TNFRSF18 \| TNFRSF1A \| TNFRSF1B \| TNFSF14 \| TNFSF18 \| TPO \| TYRO3 \| VEGFA \| XCL1
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Human Cytokine Antibody Array C1000 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer 8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AAH-CYT-1000-2.pdf

AAH-CYT-1000-2-datasheet.pdf

AAH-CYT-1000-2-safety-datasheet.pdf