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Human Cytokine Array C1

Human Cytokine Array C1

Item no.	AAH-CYT-1-2
Manufacturer	Raybiotech
Amount	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Carcinoma Hematology Leukemia Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Mesothelioma Arrays By Organ Colorectal Cancer Antibody Arrays
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Fisher, Bridget S., Carlos E. Estraño, and Judith A. Cole. "Modeling Long-Term Host Cell-Giardia Lamblia Interactions in an In Vitro Co-Culture System." PLoS One. NCBI, 03 Dec. 2013. Web.2. Caballero-Campo, Pedro, George L. Gerton, and Mariano G. Buffone. "A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis." Journal of Cellular Physiology. NCBI, 01 Jan. 2014. Web.
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	IFN-gamma, IL-10, IL-1 alpha, IL-6, IL-13, IL-8, IL-6, G-CSF, IL-2, RANTES, MCP-3, GM-CSF, IL-5, IL-7, IL-15, MCP-1, IL-3, MIG, MCP-2, GRO, TGF beta 1, TNF beta, TNF alpha, GRO alpha
Available
Short description	RayBio C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Human Cytokines. Suitable for all liquid sample types.
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected	23
Target Names	GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MIG (CXCL9), RANTES (CCL5), TGF beta 1, TNF alpha, TNF beta (TNFSF1B)
Targets Detected	CCL2, CCL5, CCL7, CCL8, CXCL1, CXCL8, CXCL9, G-CSF, GM-CSF, GRO, GRO alpha, IFN-gamma, IL-1 alpha, IL-1 F1, IL-10, IL-13, IL-15, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, MARC, MCP-1, MCP-2, MCP-3, MIG, RANTES, TGF beta 1, TNF alpha, TNF beta, TNFSF1B
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components	Human Cytokine Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Membrane
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Chemiluminescence
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	1. Fisher, Bridget S., Carlos E. Estraño, and Judith A. Cole. "Modeling Long-Term Host Cell-Giardia Lamblia Interactions in an In Vitro Co-Culture System." PLoS One. NCBI, 03 Dec. 2013. Web. 2. Caballero-Campo, Pedro, George L. Gerton, and Mariano G. Buffone. "A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis." Journal of Cellular Physiology. NCBI, 01 Jan. 2014. Web.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

AAH-CYT-1-2.pdf

AAH-CYT-1-2-datasheet.pdf

AAH-CYT-1-2-safety-datasheet.pdf