Item no. |
AAH-CYT-1-2 |
Manufacturer |
Raybiotech
|
Amount |
2 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Human (Homo sapiens) |
Host |
Hamster - Armenian |
Citations |
1. Fisher, Bridget S., Carlos E. Estraño, and Judith A. Cole. "Modeling Long-Term Host Cell-Giardia Lamblia Interactions in an In Vitro Co-Culture System." PLoS One. NCBI, 03 Dec. 2013. Web.2. Caballero-Campo, Pedro, George L. Gerton, and Mariano G. Buffone. "A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis." Journal of Cellular Physiology. NCBI, 01 Jan. 2014. Web. |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
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IFN-gamma, IL-10, IL-1 alpha, IL-6, IL-13, IL-8, G-CSF, IL-2, RANTES, MCP-3, GM-CSF, IL-5, IL-7, IL-15, MCP-1, IL-3, MIG, MCP-2, GRO, TGF beta 1, TNF beta, TNF alpha, GRO alpha |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
Number of Targets Detected |
23 |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Chemiluminescence |
Quantitative/Semi-Quantitative |
Semi-Quantitative |
Solid Support |
Membrane |
Gene Symbols |
CCL2 | CCL5 | CCL7 | CCL8 | CSF2 | CSF3 | CXCL1 | CXCL8 | CXCL9 | IFNG|Â IL10 | IL13 | IL15 | IL1A | IL2 | IL3 | IL5 | IL6 | IL7 | LTA | TGFB1 | TNF |
Kit Components |
Human Cytokine Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual |
Other Materials Required |
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline |
Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis |
Product Features |
Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex |
Storage/Stability |
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.