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Human Chemokine Array C1

Human Chemokine Array C1

Item no.	AAH-CHE-1-8
Manufacturer	Raybiotech
Amount	8 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Category	Oncology Arrays By Organ Skin Cancer Colorectal Cancer Head & Neck Cancer Bone Cancer Antibody Arrays
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. McGovern NN, Cowburn AS, Porter L, Walmsley SR, Thompson AAR, et al. Hypoxia Selectively Inhibits Respiratory Burst Activity and Killing of Staphylococcus aureus in Human Neutrophils. J Immunol. 2011,186:453–463. 2. De Boeck A., et al. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression. Proteomics. 2013 Jan,13(2):379-88. doi: 10.1002/pmic.201200179. Epub 2013 Jan 6. 3. Farahi N,Cowburn A S,Upton P D, Deighton J, Sobolewski A,et al.Eotaxin-1/CC Chemokine Ligand 11: A Novel Eosinophil Survival Factor Secreted by Human Pulmonary Artery Endothelial Cells. J Immunol. 2007,179:1264–1273 4. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1693 5. Xu Y, Ganem D. Induction of chemokine production by latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells. J Gen Virol. 2007,88:45-50. 6. Durand S H, Flacher V, Romeas A, Carrouel F,Colomb E,et al.Lipoteichoic Acid Increases TLR and Functional Chemokine Expression while Reducing Dentin Formation in In Vitro Differentiated Human Odontoblasts.J Immunol, 2006, 176: 2880-2887 7. Bingera T, Sticha S, Andreasa K, Kapsb C, Sezerc O,et al. Migration potential and gene expression profile of human mesenchymal stem cells induced by CCL25. Exp Cell Res. 2009,315:1468-1479 8. Hsu M, Wu S, Chang S, Su I, Tsai C,et al.Epstein-Barr Virus Lytic Transactivator Zta Enhances Chemotactic Activity through Induction of Interleukin-8 in Nasopharyngeal Carcinoma Cells. J. Virol. April 2008 vol. 82 no. 7 3679-3688 9. Rizzo M., et al. Low Molecular Weight Hyaluronan-Pulsed Human Dendritic Cells Showed Increased Migration Capacity and Induced Resistance to Tumor Chemoattraction. PLOS One, Published: September 19, 2014. DOI: 10.1371/journal.pone.0107944
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Available
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Detection Method	Chemiluminescence
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Kit Components	Human Chemokine Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Number of Targets Detected	38
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Result Output	Semi-Quantitative
Short description	RayBio C-Series Human Chemokine Antibody Array 1 Kit. Detects 38 Human Chemokines. Suitable for all liquid sample types.
Solid Support	Membrane
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	BLC, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CK beta 8-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL16, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, Fractalkine, GCP-2, GRO, GRO alpha, HCC-4, I-309, I-TAC, IL-8, IP-10, Lymphotactin, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MPIF-1, MPIF-2, NAP-2, PARC, PPBP, RANTES, SDF-1 alpha, SDF-1 beta, TARC, TCA-3, TCK-1, TECK, XCL1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.