Comparison

Human L182 Array, Glass Slide

Item no. AAH-BLG-ADI-1-4
Manufacturer Raybiotech
Amount 4 Sample Kit
Quantity options 4 Sample Kit 8 Sample Kit
Category
Type Array
Specific against Human (Homo sapiens)
Host Hamster - Armenian
Citations Zhong J., et al. Temporal Profiling of the Secretome during Adipogenesis in Humans. J. Proteome Res., 2010, 9 (10), pp 5228–5238
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
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Shipping condition Cool pack
Available
Number of Targets Detected
182
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Design Principle
Label-based
Solid Support
Glass Slide
Kit Components
  • RayBio L-Series Human Obesity Antibody Array Glass Slide(s)
  • Dialysis Tubes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Serum Buffer
  • Floating Dialysis Rack
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Laser scanner for fluorescence detection (list of compatible scanners available at http://www.raybiotech.com/resources.asp)
  • Aluminum foil
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis
Detection Method
Fluorescence Laser Scanner
Features
  • High density arrays
  • 182 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use
Result Output
Semi-Quantitative
Short description
RayBio L-Series Human Obesity Antibody Array 182 Glass Slide Kit. Detects 182 Human Adipokines. Suitable for serum, plasma and cell culture supernatants.
Storage
For best results, store the entire kit frozen at ‰¤ -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at ‰¤ -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
4-1BB, ACE, ACE-2, ACRP30, ACTH, ADFP, Adiponectin, Adipsin, AgRP, ALK-3, ALK-6, AMPK alpha 1, Amylin, Ang-like Factor, Angiopoietin-1, Angiopoietin-2, Angiotensin II, Angiotensinogen, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, Apelin Receptor, Apo-1, ApoB, ApoE, AXL, BDNF, beta-NGF, bFGF, BMP-15, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPR-II, C-Peptide, C-Reactive Protein, C3a des Arg, CART, CCL16, CCL18, CCL19, CCL2, CCL25, CCL3, CCL4, CCL5, CCL7, CD106, CD220, CD36, CD54, Clusterin, CNTF, Coagulation Factor III, CRP, CXCL1, CXCL12 alpha, CXCL5, CXCL8, Cystatin C, Dtk, E-Selectin, EGF, EGFR, ENA-78, Endorphin Beta, Endothelin, Epiregulin, ErbB1, FABP4, FAM3B, Fas, FGF-10, FGF-6, FSH, Galectin-1, Ghrelin, GITR, GITR Ligand, GLP-1, Glucagon, GLUT1, GLUT2, GLUT3, GLUT5, GPR-39, GPX1, GPX3, GRO alpha, Growth Hormone, HCC-4, HGF, HSD-1, Hypocretin-1, ICAM-1, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R4, IL-1 Ra, IL-10, IL-11, IL-12 p70, IL-17E, IL-25, IL-6, IL-6 R, IL-8, INSL3, INSRR, Insulin, Insulin R, Leptin, Leptin R, LIF, LOX, Luteinizing hormone, Lymphotactin, M-CSF, MARC, MCP-1, MCP-3, MIF, MIP-1 alpha, MIP-1 beta, MIP-3 beta, MMP-11, MMP-19, MMP-2, MMP-9, MSHa, MSP alpha/beta, Myostatin, NAIP, NeuroD1, Neuropeptide Y, Neuropilin-2, NGFR, NRIC3, Obestatin R, Oncostatin M, Orexin A, Orexin B, Osteocalcin, Osteonectin, Osteoprotegerin, PARC, PBEF1, PDGF, PDGF-AA, PDGF-AB, PDGF-C, PDGF-D, PEDF, Pentraxin-3, PPARg2, Pref-1, Prohibitin, Prolactin, PYY, RANTES, RBP4, RELM beta, Resistin, S100, S100 A10, S100 A8/A9, SAA, SDF-1 alpha, SEMA3A, Serotonin, Serpin F1, Serum Amyloid A, SR-B3, Stromelysin-3, Syndecan-3, TACE, TDAG51, TECK, TGF alpha, TGF beta 1, Thrombospondin 1, Thrombospondin 2, Thrombospondin 4, TIMP-1, TIMP-2, TIMP-3, TIMP-4, Tissue Factor, TLR2, TLR4, TNF alpha, TNFRSF11B, TNFRSF16, TNFRSF18, TNFRSF6, TNFRSF9, TNFSF18, TSG-14, TSG-6, TSH, Vaspin, VCAM-1, VEGF-A, Visfatin, XCL1, XEDAR

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 4 Sample Kit
Available: In stock
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