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Human L182 Array, Glass Slide

Human L182 Array, Glass Slide

Item no.	AAH-BLG-ADI-1-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Signaling Protein Aggregation, Clearance & Misfolding Neural Development Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Cell Metabolism Autophagy Endocrine Regulation of Lipid Metabolism Lipid & Fatty Acid Transport Senescence Cardiovascular Research Enzymes Other Fatty Acid Enzymes Heart Failure Myocardial Infarction Hypertension Fatty Acid Synthesis Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Renal Cancer Neurodegeneration Markers Microglia Markers Cytoskeleton Markers Antibody Arrays Other Lipid\Cholesterol Metabolism Modulators
Type	Array
Specific against	Human
Host	Hamster - Armenian
Citations	Zhong J., et al. Temporal Profiling of the Secretome during Adipogenesis in Humans. J. Proteome Res., 2010, 9 (10), pp 5228–5238
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	EGF, CRP, Adiponectin, Amylin, BDNF, bFGF, EGFR, Ghrelin, IFN-gamma, IL-10, IL-6, Insulin, Leptin, MMP-2, MMP-9, Osteoprotegerin, PEDF, TIMP-1, TIMP-3, TIMP-4, VCAM-1, TLR2, TLR4, BMP-7, ANGPTL2, IL-12, IL-8, IL-6, SDF-1, Resistin, ANGPTL1, BMP-2, HGF, MIF, LIF, ANGPTL4, IGFBP-2, Serotonin, CNTF, VEGF-A, Epiregulin, PDGF-C, Prohibitin, Prolactin, TACE, PARC, NAIP, XEDAR, Clusterin, S100, RBP4, ANGPTL3, FABP4, FAM3B, IGF-1, Axl, RANTES, Glucagon, ICAM1, SEMA3A, INSL3, OSM, TGF-b, M-CSF, MIP-1 alpha, MCP-3, SAA, TSH, FSH, IL-6 sR, IL-11, MCP-1, BMP-3, ENA-78, GLP-1, Lymphotactin, Myostatin, TECK, TIMP-2, Vaspin, PDGF-AA, BMP-5, BMP-4, MIP-1 beta, PDGF, PDGF-AB, IL-1a, IL-1b, PYY, CD36, LOX, E-selectin, Osteonectin, Glutathione peroxidase 3, CART, HSD-1, Osteocalcin, Syndecan-3, TDAG51, INSRR, ACTH, MIP-3b, IGFBP-1, Cystatin C, ADFP, FGF-10, FGF-6, BMP-6, TNF alpha, Glutathione peroxidase 1, IGFBP-3, Angiopoietin 2, ApoB, Orexin A, MMP-11, NeuroD1, TGF alpha, Thrombospondin 2, PDGF-D, Orexin B, IGF-2, Leptin R, CD137 (4-1BB), GITR, BMPR-II, Pref-1, RELMb, TSG-6, Dtk, ApoE, AgRP, Glut2, Thrombospondin 1, sTNFRI, IL-1 ra, sTNFRII, IL-1 R4, IL-1 R1, GITR Ligand, GRO alpha, C-peptide, b-NGF, NGF R, Angiopoietin 1, IGF-1 sR, ACE-2, ACE / CD43, Adipsin (Factor D), AMPKa1, Angiotensinogen / Angiotensin II, Ang-like Factor, Apelin Receptor, BMP-3b / GDF-10, BMP-8, BMP-15, BMPR-IA / ALK-3, BMPR-IB / ALK-6, C3a des Arg, Endophin Beta, ET-1 (Endothelin), FAS / Apo-1, Galectin -1, Growth Hormone (Growth Hormone), Glut1, Glut3, Glut5, HCC4, IL-25 / IL-17E, Insulin R (CD220), LH (Luteinizing Hormone), MMP-19, MSHa, MSPa, Neurophilin-2, NPY (Neuropeptide Y), Obestatin R (GPR-39), Pentraxin-3, PPARg2 / NRIC3, S100 A8+A9, S100 A10, Thrombospondin 4, Tissue factor (CD142), Visfatin/PBEF1
Available
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Label-based
Detection Method	Fluorescence Laser Scanner
Features	High density arrays 182 Human proteins Direct biotin labeling of proteins High detection sensitivity Accurate and reproducible Affordable, quick and simple to use
Kit Components	RayBio L-Series Human Obesity Antibody Array Glass Slide(s) Dialysis Tubes Labeling Reagent Stop Solution Blocking Buffer Wash Buffer 1 Wash Buffer 2 Streptavidin-Conjugated Fluor Adhesive Plastic Strips Serum Buffer Floating Dialysis Rack 30 ml Centrifuge Tube
Number of Targets Detected	182
Other Materials Required	Distilled or de-ionized water KCl, NaCl, KH2PO4 and Na2HPO4 Small plastic or glass containers Orbital shaker or oscillating rocker Beaker, stir plate and stir bar 1 ml tube Pipettors, pipette tips and other common lab consumables Laser scanner for fluorescence detection (list of compatible scanners available at http://www.raybiotech.com/resources.asp) Aluminum foil
Protocol Outline	Dialyze samples in PBS Biotinylate Samples Dialyze biotinylated samples using spin column Dry the glass slide Block array surface Incubate with Samples Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Result Output	Semi-Quantitative
Short description	RayBio L-Series Human Obesity Antibody Array 182 Glass Slide Kit. Detects 182 Human Adipokines. Suitable for serum, plasma and cell culture supernatants.
Solid Support	Glass Slide
Storage	For best results, store the entire kit frozen at ‰¤ -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at ‰¤ -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	4-1BB, ACE, ACE-2, ACRP30, ACTH, ADFP, Adiponectin, Adipsin, AgRP, ALK-3, ALK-6, AMPK alpha 1, Amylin, Ang-like Factor, Angiopoietin-1, Angiopoietin-2, Angiotensin II, Angiotensinogen, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, Apelin Receptor, Apo-1, ApoB, ApoE, AXL, BDNF, beta-NGF, bFGF, BMP-15, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMPR-II, C-Peptide, C-Reactive Protein, C3a des Arg, CART, CCL16, CCL18, CCL19, CCL2, CCL25, CCL3, CCL4, CCL5, CCL7, CD106, CD220, CD36, CD54, Clusterin, CNTF, Coagulation Factor III, CRP, CXCL1, CXCL12 alpha, CXCL5, CXCL8, Cystatin C, Dtk, E-Selectin, EGF, EGFR, ENA-78, Endorphin Beta, Endothelin, Epiregulin, ErbB1, FABP4, FAM3B, Fas, FGF-10, FGF-6, FSH, Galectin-1, Ghrelin, GITR, GITR Ligand, GLP-1, Glucagon, GLUT1, GLUT2, GLUT3, GLUT5, GPR-39, GPX1, GPX3, GRO alpha, Growth Hormone, HCC-4, HGF, HSD-1, Hypocretin-1, ICAM-1, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R4, IL-1 Ra, IL-10, IL-11, IL-12 p70, IL-17E, IL-25, IL-6, IL-6 R, IL-8, INSL3, INSRR, Insulin, Insulin R, Leptin, Leptin R, LIF, LOX, Luteinizing hormone, Lymphotactin, M-CSF, MARC, MCP-1, MCP-3, MIF, MIP-1 alpha, MIP-1 beta, MIP-3 beta, MMP-11, MMP-19, MMP-2, MMP-9, MSHa, MSP alpha/beta, Myostatin, NAIP, NeuroD1, Neuropeptide Y, Neuropilin-2, NGFR, NRIC3, Obestatin R, Oncostatin M, Orexin A, Orexin B, Osteocalcin, Osteonectin, Osteoprotegerin, PARC, PBEF1, PDGF, PDGF-AA, PDGF-AB, PDGF-C, PDGF-D, PEDF, Pentraxin-3, PPARg2, Pref-1, Prohibitin, Prolactin, PYY, RANTES, RBP4, RELM beta, Resistin, S100, S100 A10, S100 A8/A9, SAA, SDF-1 alpha, SEMA3A, Serotonin, Serpin F1, Serum Amyloid A, SR-B3, Stromelysin-3, Syndecan-3, TACE, TDAG51, TECK, TGF alpha, TGF beta 1, Thrombospondin 1, Thrombospondin 2, Thrombospondin 4, TIMP-1, TIMP-2, TIMP-3, TIMP-4, Tissue Factor, TLR2, TLR4, TNF alpha, TNFRSF11B, TNFRSF16, TNFRSF18, TNFRSF6, TNFRSF9, TNFSF18, TSG-14, TSG-6, TSH, Vaspin, VCAM-1, VEGF-A, Visfatin, XCL1, XEDAR

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