Item no. |
AAH-ATH-1-4 |
Manufacturer |
Raybiotech
|
Amount |
4 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Category |
|
Type |
Array |
Specific against |
Human (Homo sapiens) |
Host |
Hamster - Armenian |
Citations |
1. Woroniecki R P, Shatat I F, Supe K, Du Z F, Frederick J K.Urinary Cytokines and Steroid Responsiveness in Idiopathic Nephrotic Syndrome of Childhood. Am J Nephrol. 2008,28:83–90 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IFN-gamma, IL-1 alpha, IL-1 beta, G-CSF, GDNF, RANTES, M-CSF, GM-CSF, MCP-1, ICAM-1, PDGF-BB, Eotaxin-1, TGF beta 1, TGF beta 2, TGF beta 3, TNF beta, MIP-3 alpha, TNF alpha, IL-1 sRI, IL-1 sRII |
Shipping condition |
Cool pack |
Available |
|
Number of Targets Detected |
20 |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Solid Support |
Membrane |
Kit Components |
- Human Atherosclerosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Detection Method |
Chemiluminescence |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Result Output |
Semi-Quantitative |
Short description |
RayBio C-Series Human Atherosclerosis Antibody Array 1 Kit. Detects 20 Human Atherosclerotic Factors. Suitable for all liquid sample types. |
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
CCL11, CCL2, CCL20, CD54, Eotaxin-1, G-CSF, GDNF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R1, IL-1 R2, M-CSF, MCP-1, MIP-3 alpha, PDGF-BB, RANTES, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF beta, TNFSF1B |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.