Comparison

Human Obesity Array C1

Item no. AAH-ADI-1-8
Manufacturer Raybiotech
Amount 8 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Category
Type Array
Specific against Human
Host Hamster - Armenian
Citations Bauer D., Mazzio E., Soliman K., Taka E., Oriaku E., et al. Diallyl Disulfide Inhibits TNFalpha-induced CCL2 Release by MDA-MB-231 Cells. ANTICANCER RESEARCH 34: 2763-2770 (2014)
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
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Available
Number of Targets Detected
62
Species Detected
Human
Compatible Sample Types
Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates
Design Principle
Sandwich-based
Method of Detection
Chemiluminescence
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
Membrane
Gene Symbols
ACE2 | ADIPOQ | AGRP | ANGPT1 | ANGPT2 | ANGPTL4 | CCL16 | CCL18 | CCL2 | CCL25| CCL4 | CCL5 | CCL7 | CFD | CRP | CSF1 | CXCL10 | CXCL12 | CXCL5 | CXCL8 | EDA2R | FAS | FGF6 | GH1 | IFNG | IGF1 | IGF1R | IGFBP1 | IGFBP2 | IGFBP3 | IL10 | IL11 | IL12A | IL1A | IL1B | IL1R1 | IL1RL1 | IL6 | IL6R | INS | LEP | LEPR | LIF | MIF | MST1 | OSM | PDGFA | PDGFB | RETN | SAA1 | SERPINE1 | TGFB1 | TIMP1 | TIMP2 | TNF | TNFRSF11B | TNFRSF1A | TNFRSF1B | TNFRSF9 | TNFSF9 | VEGFA | XCL1
Application Information
Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker/ Key Factor Screening
Identifying Key Factors
Confirming a Biological Process
Kit Components
Human Obesity Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-
Conjugated H
R
P Detection Buffer C Detection Buffer D Lysis Buffer 8-
Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U
V
P's Chemi
Doc-
It® or Epi
Chem I
I Benchtop Darkroom or G
E's Image
Quant™ L
A
S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H
R
P-
Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features
Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 8 Sample Kit
Available: In stock
available

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