Item no. |
QAE-CYT-1-1 |
Manufacturer |
Raybiotech
|
Amount |
8 Sample Kit |
Category |
|
Type |
Assay |
Specific against |
Equine |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IL-10, IL-1 alpha, VEGF, IL-4, IL-8, IL-2, IFN gamma, IL-15, MCP-1, IL-1R alpha |
Available |
|
Short description |
Quantibody Equine (horse) Cytokine Array 1 Kit. Detects 10 Equine Cytokines. Suitable for all liquid sample types. |
Features |
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 uL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
|
Number of Targets Detected |
10 |
Target Names |
IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 Ra (IL-1 F3), IL-2, IL-4, IL-8 (CXCL8), IL-10, IL-15, MCP-1 (CCL2), VEGF-A |
Targets Detected |
CCL2, CXCL8, IFN-gamma, IL-1 alpha, IL-1 F1, IL-1 F3, IL-1 Ra, IL-10, IL-15, IL-2, IL-4, IL-8, MCP-1, VEGF-A |
Suggested Applications |
Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range |
Kit Components |
- Quantibody Bovine Cytokine Array 1 Slide
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
|
Other Materials Required |
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Glass Slide |
Design Principle |
Sandwich-based |
Result Output |
Quantitative |
Detection Method |
Fluorescence Laser Scanner |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.