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Human SFRP2 Protein (SRP-HM102)

Human SFRP2 Protein (SRP-HM102)

Item no.	SRP-HM102-100UG
Manufacturer	KactusBio
Amount	100 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Lyophilized
Specific against	Human
Host	HEK293
Conjugate/Tag	HIS
Purity	> 95% as determined by Tris-Bis PAGE
Formula	Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 7.4).
Sequence	Leu25-Cys295
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	sFRP-2; SARP-1; Sarp1; Sdf5; Sfrp2; AI851596
Available
Description	Recombinant Human SFRP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Cys295.
Background	As biomarkers, DNA methylation is used to detect colorectal cancer (CRC) and make assessment of CRC prognosis. The published findings showed the association between the methylation of SFRP1, SFRP2, and WIF1, located in the Wnt signaling pathway, and the prognosis of CRC were not consistent. SFRP1, SFRP2, and WIF1 were frequently hypermethylated in CRC tumor tissues. It was apparent that the promoter hypermethylation of SFRP2 and co-hypermethylation of SFRP1 and SFRP2 might be considered as independent prognostic predictors for survival advantage of postoperative CRC patients.
Manufacturers Product Type	Recombinant Protein
Endotoxin	Less than 1EU per μg by the LAL method.
Molecular Weight	The protein has a predicted MW of 32.4 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Tris-Bis PAGE result.
Stability and Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3-6 months after reconstitution.；2-8°C for 2-7 days after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22μm filtered solution in 20mM PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Tag Information	C-His
Formulation	Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 7.4).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

SRP-HM102-100UG-datasheet.pdf

SRP-HM102-100UG-safety-datasheet.pdf