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Human Siglec-10 Protein, Fc (L118A, G120A, E201A) Tag

Human Siglec-10 Protein, Fc (L118A, G120A, E201A) Tag

Item no.	SI0-H5255-1mg
Manufacturer	ACROBiosystems
Amount	1 mg
Category	Peptides & Proteins Amino Acids
Type	Proteins
Format	Powder
Specific against	Human
Host	HEK293
Conjugate/Tag	Unconjugated, Fc
Purity	85%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	SIGLEC10,MGC126774,PRO940,Siglec10,SLG2
Available
Manufacturers Category	Protein / Bio-Markers & CD Antigens
Description	Human Siglec-10, Fc (L118A, G120A, E201A) Tag (SI0-H5255) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Molecule	Siglec-10
Exp Region	Met 17 - Thr 546
Storage	-20℃
Shipping	RT
Stability	-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Molecular Weight	84.6 kDa
Characteristics	This protein carries a human IgG1 Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. As the result of the mutations for Fc-Tag, the protein migrates as 90-110 kDa (Siglect-10 with Fc-Tag), 60-67 kDa (Siglect-10) and 30-32 kDa (Fc fragment) under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
Endotoxin	1.0 EU per μg
Buffer	PBS, pH7.4
Background	The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 1 mg

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Delivery expected until 8/16/2024

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