« Back
Home /
Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag™ (BA.2/Omicron)

Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag™ (BA.2/Omicron)

Item no.	S2N-C82E4-200ug
Manufacturer	ACROBiosystems
Amount	200 ug
Quantity options	200 ug 25 ug
Category	Peptides & Proteins Amino Acids
Type	Proteins Recombinant
Format	Powder
Specific against	SARS-CoV-2
Host	HEK293
Conjugate/Tag	HIS, Avi, Biotin
Purity	95%
NCBI	QHD43416.1 (N764K, D796Y, Q954H, N969K, F817P, A892P, A899P, A942P, K986P, V987P)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2
Shipping condition	Room temperature
Available
Manufacturer - Category	Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag	C-10×His & C-Avi, Biotin-labeled
Shipping Temperature	RT
Storage Conditions	-20°C
Molecular Weight	61.7 kDa
Description	Biotinylated SARS-CoV-2 Spike S2 protein, His, Avitag™ (BA.2/Omicron) (S2N-C82E4) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1 (N764K, D796Y, Q954H, N969K, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2/3/4/5). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.
Background	It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Molecule	Spike S2
Exp Region	Ser 686 - Pro 1213
Characteristics	This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 61.7 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin	1.0 EU per μg
Buffer	PBS, pH7.4
Stability	● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant	trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

S2N-C82E4-200ug-datasheet.pdf

S2N-C82E4-200ug-safety-datasheet.pdf