Recombinant Human Ubiquitin (K27 only) protein

Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 μM. In Ub(K27 only), all lysine residues but lysine 27 were substituted with arginines, thereby it only supports the formation of lysine 27-linked polyubiquitin chains.

Proteins

Ubiquitination related reagent

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol