« Back
Home /
Recombinant Human EphA3 Protein

Recombinant Human EphA3 Protein

Manufacturer	Abclonal
Category	Oncology By Cell Type Hematology Leukemia Myeloma Peptides & Proteins Recombinant Proteins Myelofibrosis
Type	Proteins Recombinant
Specific against	Human
Amount	10 ug
Host	Human
Item no.	RP00186-10ug
Targets	EPHA3;;EPHA5
eClass 6.1	34160400
eClass 9.0	42020190
Available
Alias	EK4, ETK, ETK1, HEK, HEK4, TYRO4
Purity	> 95% by SDS-PAGE.
Description	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Background	EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis. It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue . EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration. Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5. It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A.
Route	C-hFc&His
Manufacturers Category	Proteins
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Immunogen	Met1-Gln541
Storage	Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Manufacturers Research Area	Biosimilar Drug Targets
Gene Symbol	EphA3
Protein Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Protein Description	Recombinant Human EphA3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Gln541) of human EphA3 (Accession #NP_005224.2) fused with an Fc, 6×His tag at the C-terminus.
Protein Bio Activity	Measured by its binding ability in a functional ELISA.Immobilized Human EFNA5 at 0.5μg/mL (100 μL/well) can bind Human EPHA3 with a linear range of 0.01-3.9 ng/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 10 ug

available

Delivery expected until 8/23/2024

Express Delivery: Save 24 days

Compare

Add to wishlist

Recommend by mail