Item no. |
PRS-97-076-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized |
Applications |
WB |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
IL2, TCGF, lymphokine, Interleukin 2 |
Available |
|
Applications |
This recombinant protein can be used for WB. For research use only. |
Applications |
This recombinant protein can be used for WB. For research use only. |
Buffer |
Lyophilized from 0.22 um filtered solution in PBS, pH5.0. Normally trehalose is added as protectant before lyophilization |
Storage Conditions |
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
15.4 kDa |
Purity |
>95% as determined by SDS-PAGE. |
Background |
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system. |
1St Image Caption3 |
Human IL-2, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. |
Background References 1 |
Gaffen, S.L. and K.D. Liu, 2004, Cytokine 28:109. |
Background References 2 |
Kasakura S, Lowenstein L, 1965, Nature 208 (5012): 794–795. |
Background References 3 |
Smith KA, Favata MF, Oroszlan S, 1983, J. Immunol. 131 (4): 1808. |
Background References 4 |
Thornton AM, Shevach EM, 1998, J. Exp. Med. 188 (2): 287–96. |
Accession # |
P60568 |
Ncbi Gene Id # |
3558 |
Ncbi Official Symbol |
IL2 |
Ncbi Official Full Name |
interleukin 2 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P60568 |
Fusion Tag |
Tag free |
Sequence |
Ala 21 - Thr 153 |
Biological Activity |
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse CTLL-2 cells. The ED50 for this effect is 0.284-0.355 ng/ml (Routinely tested). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.