Item no. |
PRS-95-103-0.05mg |
Manufacturer |
ProSci
|
Amount |
0.05 mg |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
other |
Dry ice |
Yes
|
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
EndoG Antibody : Endo G |
Available |
|
Applications |
This recombinant protein can be used for WB and ELISA. For research use only. |
Applications |
This recombinant protein can be used for WB and ELISA. For research use only. |
Buffer |
1X PBS containing 0.1% SDS and 0.02% NaN3 |
Storage Conditions |
Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
15 kDa (Calculated) |
Purity |
~95% |
Background |
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4). |
Related Products |
EndoG Antibody, Catalog No. 3035.EndoG Antibody (7G1C10), Catalog No. 4577.EndoG Antibody (7G1G10), Catalog No. 4579.EndoG Antibody (7F2G7), Catalog No. 4581.EndoG Antibody (7F2G10), Catalog No. 4583.DFF40 Antibody (I18), Catalog No. 2107. DFF45 Antibody (CT), Catalog No. 1141. |
1St Image Caption3 |
SDS-PAGE analysis of recombinant EndoG fragment on Coomassie Blue-stained 4-20% gradient gel. |
Background References 1 |
Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9. |
Background References 2 |
Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans . Nature 2001; 412:90-4 |
Background References 3 |
Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29. |
Background References 4 |
Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9. |
Accession # |
Q14249 |
Ncbi Gene Id # |
2021 |
Ncbi Official Symbol |
ENDOG |
Ncbi Official Full Name |
endonuclease G |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14249 |
Fusion Tag |
Fusion Partner: His-tag and strepII-tag at N-terminus |
Sequence |
aa. 51 - 140 |
Biological Activity |
N/A |
Protein Gi# |
24638471 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.