Comparison

CD152 [CTLA-4] Recombinant Protein

Item no. PRS-90-415-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Proteins Recombinant
Format Lyophilized
Specific against other
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CTLA-4
Available
Applications
This recombinant proteins is for research use only.
Applications
This recombinant proteins is for research use only.
Buffer
Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μ l sterile water. Add 1X PBS to the desired protein concentration.
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C. Working aliquots are stable for up to 3 months when stored at -20˚ C.
Disclaimer
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Purity
>98% (SDS-PAGE).

Endotoxin level is less than 0.06EU/ μ g protein (LAL test; Lonza).
Background
CD152 [CTLA-4] and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD152 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD152 and CD28 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD152 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. CD152 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CD152 transcripts have since been found in both Th1 and Th2, and CD4 + and CD8 + T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4 + T cells and 50% of CD8 + T cells and is down regulated upon T cell activation, CD152 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CD152 and CD28 can bind to the same ligands, CD152 binds to B71 and B72 with 20-100-fold higher affinity than CD28.
Accession #
NP_005205
Ncbi Gene Id #
1493
Ncbi Official Symbol
CTLA4
Ncbi Official Full Name
cytotoxic T-lymphocyte associated protein 4
Ncbi Organism
Homo sapiens
Swissprot #
P01584
Fusion Tag
Fc Tag
Sequence
The extracellular domain of human CD152 [CTLA-4] (aa 37-160) is fused to the N-terminus of the Fc region of mouse IgG2a.
Biological Activity
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells.
Protein Gi#
21361212

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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