Item no. |
PRS-40-289-0.2mg |
Manufacturer |
ProSci
|
Amount |
0.2 mg |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
OB, OBS, LEPD, OB, Leptin, Obese protein |
Available |
|
Storage Conditions |
The lyophilized Leptin recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted Leptin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing Leptin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Disclaimer |
This product is for research use only. |
Purity |
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μ g (1EU/μ g). |
Background |
Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant human Leptin is a 16.0 kDa protein containing 147 amino acid residues. |
Accession # |
NP_000221.1 |
Ncbi Gene Id # |
3952 |
Ncbi Official Symbol |
LEP |
Ncbi Official Full Name |
leptin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P41159 |
Sequence |
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC |
Biological Activity |
Human Leptin is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 μ g Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline - treated controls. |
Protein Gi# |
4557715 |
Peptide Sequence |
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.