Item no. |
PRS-11-323-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
TIGIT,VSIG9,VSTM3 |
Available |
|
Applications |
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.1 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Applications |
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.1 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Buffer |
PBS, pH7.4 |
Storage Conditions |
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
23.1 kDa |
Purity |
>90% as determined by SDS-PAGE. |
Background |
TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells. |
1St Image Caption3 |
Canine TIGIT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. |
Background References 1 |
Chew GM., et al., 2016, PLoS Pathogens., 12: e1005349. |
Background References 2 |
Joe-Marc Chauvin, et al., 2015, J Clin Invest., 125 (5): 2046-2058. |
Background References 3 |
Steven G. Deeks, 2012, Nature, 487 (1): 439-440. |
Fusion Tag |
His Tag |
Sequence |
Phe 17 - Pro 208 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.