IL-8 / CXCL8 Recombinant Protein

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.8 kDa. The protein migrates as 37-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

34.8 kDa

Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

Hedges JC. 2000. Am J Respir Cell Mol Biol. 23:86-94.

Hébert CA. 1990. J Immunol. 145(9):3033-40.

3576

C-X-Cmotifchemokineligand8

P10145

Ser 28 - Ser 99