Item no. |
PRS-11-219-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CXCL8,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1 |
Available |
|
Applications |
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.8 kDa. The protein migrates as 37-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Applications |
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.8 kDa. The protein migrates as 37-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Buffer |
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 |
Storage Conditions |
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
34.8 kDa |
Purity |
>95% as determined by SDS-PAGE. |
Background |
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. |
1St Image Caption3 |
Human IL-8, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. |
Background References 1 |
Hedges JC. 2000. Am J Respir Cell Mol Biol. 23:86-94. |
Background References 2 |
Van Damme J. 1990. Eur J Immunol. 20(9):2113-8. |
Background References 3 |
Hébert CA. 1990. J Immunol. 145(9):3033-40. |
Accession # |
NP_000575.1 |
Ncbi Gene Id # |
3576 |
Ncbi Official Symbol |
CXCL8 |
Ncbi Official Full Name |
C-X-Cmotifchemokineligand8 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P10145 |
Fusion Tag |
Fc Tag |
Sequence |
Ser 28 - Ser 99 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.