Item no. |
PRS-11-196-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Proteins Recombinant |
Format |
Lyophilized |
Applications |
WB |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1,COVID-19 |
Available |
|
Applications |
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation. |
Applications |
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation. |
Buffer |
PBS, pH7.4 |
Storage Conditions |
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
58.8 kDa |
Purity |
>90% as determined by SDS-PAGE. |
Background |
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
1St Image Caption3 |
HCoV-229E S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. |
Background References 1 |
Wan Y, et al. J Virol. 2020. pii: JVI.00127-20. |
Background References 2 |
Benvenuto D, et al. J Med Virol. 2020. |
Background References 3 |
Chang CY, et al. AMB Express. 2020. 10(1):20. |
Fusion Tag |
His Tag |
Sequence |
Cys 16 - Ala 536 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.