Comparison

MERS Nucleocapsid protein, His Tag European Partner

Item no. NUN-M52H5-100ug
Manufacturer ACROBiosystems
Amount 100 ug
Quantity options 100 ug 1 mg
Category
Type Proteins Recombinant
Format Powder
Specific against MERS-CoV
Host HEK293
Conjugate/Tag HIS, Unconjugated
Purity 90%
Citations (1) Reuter M, et al. Virus Genes. 2018. 54(1):5-16.
(2) Grunewald ME, et al. Virology. 2018. 517:62-68.
(3) Jeeva S, et al. PLoS One. 2017. 12(9):e0184935.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Nucleocapsid protein,NP,Protein N,COVID-19
Shipping condition Room temperature
Available
Specificity MERS
Manufacturer - Category
Protein / Viral Proteins
Manufacturer - Conjugate / Tag
C-6×His, Unconjugated
Manufacturer - Species
MERS
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
46.7 kDa
Description
MERS Nucleocapsid protein, His Tag (NUN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Asp 413 (Accession # K0BVN3-1).
Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
Molecule
Nucleocapsid protein
Exp Region
Met 1 - Asp 413
Characteristics
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin cleavage site,  251-RHKR-254,  and is partially processed into N and C-terminal fragment with calculated MW of 17.1 kDa and 19.7 kDa respectively. The protein migrates as 23-25 kDa (C-terminus) and 46-73 kDa (full length) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
PBS, pH7.4
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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