[Novoprotein] Recombinant E. coli G/U Mismatch-Specific DNA Glycosylase/Mug (C-6His)

E. coli Mismatch Uracil DNA Glycosylase (Mug protein) is an 18 kDa constitutively expressed protein which belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine & removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. Uracil bases in DNA can arise from deamination of cytosine giving rise to increased spontaneous mutations. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA & the mispaired base. The complementary strand guanine functions in substrate recognition. It is required for DNA damage lesion repair in stationary-phase cells.

Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

19, 74

Met1-Arg168

Store at < -20C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.