Item no. |
NKM-PPH0901-100ug |
Manufacturer |
NKMAX
|
Amount |
100ug |
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Conjugate/Tag |
Non-Tagged |
Purity |
> 95% by SDS - PAGE |
Sequence |
MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM |
NCBI |
NP_006338 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Peptidylprolyl isomerase H, Peptidylprolyl isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, SnuCyp-20, PPIase H, Rotamase H, Cyclophilin H, Peptidylprolyl isomerase H CypH, EC 5.2.1.8, MGC5016, Small nuclear ribonucleoprotein particle-specific cyclophilin H, uSACYP, usnRNP associated cyclophilin SnuCyp20, |
Available |
|
Activity |
Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
Antigen Species |
Human |
Applications info |
Enzyme Activity, SDS-PAGE |
Biological activity |
Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. |
Concentration |
1 mg/ml (determined by Bradford assay) |
Description |
Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human u4/u6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the u4/u6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques. |
Domain |
1-177aa |
Endotoxin |
< 1.0 EU per 1ug of protein (determined by LAL method) |
Expression System |
E.coli |
Formulation |
Liquid in Phosphate buffered saline (pH7.4), 10% glycerol |
Gene Category |
Cell Cycle |
General References |
Reidt u., et al. (2000) J Biol Chem. 275(11):7439-42. Horowitz DS., et al. (2002) EMBO. 21(3):470-80. |
Molecular Weight |
19.2 kDa (177 aa), confirmed by MALDI-TOF. |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.