Recombinant human Leptin/OB protein

SDS-PAGE

Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Recombinant human leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

< 1.0 EU per 1ug of protein (determined by LAL method)

Liquid. In Phosphate buffered saline, pH 7.4.

Jeong KJ., et al. (1999) Appl Environ Microbiol. 65(7), 3027-32.
Anini Y., et al. (2003) Diabetes. 52(2), 252-259.
Hyogo H., et al. (2002) J. Biol. Chem. 277(37), 34117-34124.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.