Recombinant human Hexokinase 1 protein

Specific activity is > 8, 000pmol/min/ug. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37C.

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Jon E. et al., (2003) J.Exp Biology. 206 : 2049-2057.; ; Furuta H. et al., (1996) Genomics. 36(1):206-9.; ;

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.