Item no. |
NKM-ATGP4086-10ug |
Manufacturer |
NKMAX
|
Amount |
10ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid in. 50mM MES buffer (pH 5.5) containing 100mM NaCl, 20% glycerol. |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 95% by SDS-PAGE |
Citations |
Fusek M, et al. (2005). 149: 43-50.<br> Minarowska A, et al. (2007)l. 45: 159-163.<br> Tsukuba, et al. (2000) Mol. Cells 10:601-611.<br> |
NCBI |
NP_001900.1 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CPSD, CTSD, ctsd, Cathepsin D, CLN10, HEL-S-130P, lysosomal aspartyl protease, lysosomal aspartyl peptidase, lysosomal aspartyl protease, neuronal 10 |
Available |
|
Antigen Species |
Human |
Bioactivity Comment Only |
Specific activity is > 20 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 3.5 at 25C. |
Bioactivity Comment and Pics |
Specific activity is > 20 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 3.5 at 25C. |
Bioassay Procedure |
LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL |
Concentration |
1 mg/ml (determined by Absorbance at 280nm) |
Description |
Cathepsin D, also known as lysosomal aspartyl protease, is a member of the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. The main physiological functions of cathepsin D consist of metabolic degradation of intracellular proteins, activation and degradation of polypeptide hormones and growth factors, activation of enzymatic precursors, processing of enzyme activators and inhibitors, brain antigen processing and regulation of programmed cell death. In addition, it secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases. Recombinant human Cathepsin D, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
Domain |
21-412aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
HEK293 |
Molecular Weight |
43.4 kDa (398aa) |
Predicted nterm |
Leu21 |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.