Recombinant human CCL2/JE/MCP-1 protein

Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/ml.

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

CCL2(Chemokine ligand 2), as known as MCP-1(monocyte chemotactic protein-1), is a small cytokine belonging to the CC chemokine family. This protein recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It binds to chemokine receptors CCR2 and CCR4. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. Recombinant human CCL2/JE/MCP-1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Immunology, Hormones & Cytokines

9.5kDa (82aa)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.