Item no. |
NKM-ATGP4052-20ug |
Manufacturer |
NKMAX
|
Amount |
20ug |
Category |
|
Type |
Proteins |
Applications |
SDS-PAGE, Bioactivity |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
>90% by SDS-PAGE |
NCBI |
NP_033289.2 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
secreted phosphoprotein 1, 2AR, Ap1-1, BNSP, Bsp, BSP1, Eta, ETA-1, mi, OP, Opn, Opn1, Spp-1, osteopontine isoform 4, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Osteoponitin, Spp1 |
Available |
|
Antigen Species |
Mouse |
Bioactivity Comment Only |
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to mouse SPP1 coated plates. The ED50 range ≤ 1.5 ug/ml. |
Bioactivity Comment and Pics |
Mouse SPP1 stimulates cell adhesion of the HEK293 human embryonic kidney cells. The ED50 range ≤ 1.5 ug/ml. |
Bioassay Procedure |
LPVKVTD SGSSEEKLYS LHPDPIATWL VPDPSQKQNL LAPQNAVSSE EKDDFKQETL PSNSNESHDH MDDDDDDDDD DGDHAESEDS VDSDESDESH HSDESDETVT ASTQADTFTP IVPTVDVPNG RGDSLAYGLR SKSRSFQVSD EQYPDATDED LTSHMKSGES KESLDVIPVA QLLSMPSDQD NNGKGSHESS QLDEPSLETH RLEHSKESQE SADQSDVIDS QASSKASLEH QSHKFHSHKD KLVLDPKSKE DDRYLKFRIS HELESSSSEV N |
Concentration |
0.25mg/ml (determined by Absorbance at 280nm) |
Description |
Osteopontin, also known as SPP1, is major non-collagenous bone protein that binds tightly to hydroxyapatite. This protein appears to form an integral part of the mineralized matrix. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration. Osteopontin acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. Also it is essential in the pathway that leads to type 1 immunity. It is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Recombinant mouse Osteopontin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
Domain |
17-294aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
HEK293 |
Formulation |
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Category |
Angiogenesis, Cardiovascular, Adhesion & Movement |
General References |
Icer MA, et al, (2018) Clin Biochem. 51:17-24.; ; Kaleta B. (2019) Inflamm Res. 68:93-102.; ; Lamort AS, et al, (2019) Cells. 8:815.; ; |
Molecular Weight |
31.8kDa (287aa) |
Predicted nterm |
Asp |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.