Recombinant mouse Thrombospondin-4 protein

QATPQVFDLL PSSSQRLNPS ALQPVLTDPT LHEVYLISTF KLQSKSSATI FGLYSSSDNS KYFEFTVMGR LNKAILRYLK NDGKIHLVVF NNLQLADGRR HRVLLRLSNL QRGDGSVELY LDCAQADSVR NLPRAFSGLT QNPESIELRT FQRKPQDFLE ELKLVVRGSL FQVASLQDCF LQQSEPLAAT STGDFNRQFL GQMTQLNQLL GEVKDLLRQQ VKETSFLRNT IAECQACGPL SFQSPTPNTL VPIAPPAPPT RPTRHCDSSP CFRGVRCTDT RDGFQCGPCP DGYTGNGITC SDVDECKYHP CYPGVRCVNL APGFRCDACP VGFTGPMVQG VGINFAKTNK QVCTDVDECQ NGACVLNSIC INTLGSYRCG PCKPGYTGDQ TRGCKTERSC RNPEQNPCSV HAQCIEERQG DVTCVCGVGW AGDGYVCGKD VDIDSYPDEE LPCSARNCKK DNCKYVPNSG QEDADRDGIG DACDEDADGD GILNEQDNCV LTHNIDQRNS DKDIFGDACD NCRMVLNNDQ KDTDGDGRGD ACDDDMDGDG IKNILDNCPR VPNRDQQDRD GDDVGDACDS CPDVSNPNQS DVDNDLVGDS CDTNQDSDGD GHQDSTDNCP TVINSSQLDT DKDGIGDECD DDDDNDGIPD LVPPGPDNCR LVPNPAQEDS NNDGVGDICE ADFDQDQVID HIDVCPENAE ITLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTQTDDD YAGFIFGYQD SSSFYVVMWK QTEQTYWQAT PFRAVAEPGI QLKAVKSKTG PGEHLRNSLW HTGDTSDQVR LLWKDSRNVG WKDKVSYRWF LQHRPQVGYI RVRFYEGSEL VADSGVTIDT TMRGGRLGVF CFSQENIIWS NLKYRCNDTI PEDFQEFQTQ SFDRLDN

Thrombospondin-4, also known as THBS4, is a member of the thrombospondin protein family. This family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. Among them Thrombospondin-4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin‑1, Fibronectin, and Matrilin‑2. And it plays a role in cardiovascular physiology and neuronal development. It is up‑regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia. It is also up‑regulated in muscle following denervation. It contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature. Recombinant mouse Thrombospondin-4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 0.1mM PMSF, 20% glycerol

Lawler, J. et al. (1995) J. Biol. Chem. 270:2809-2814.; ; Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110-37117.; ; Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083-1094.; ; Muppala S. et al.(2015) Arteriosclerosis. 1975-1986.; ;

Gln27