Item no. |
NKM-ATGP4029-250ug |
Manufacturer |
NKMAX
|
Amount |
250ug |
Category |
|
Type |
Proteins |
Applications |
SDS-PAGE, Bioactivity |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 90% by SDS - PAGE |
NCBI |
NP_034688 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
iinterleukin 5, Il, Il-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, B-cell differentiation factor I, EDF, IL5, TRFB cell differentiation factor I |
Available |
|
Antigen Species |
Mouse |
Bioactivity Comment Only |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 ng/ml. |
Bioactivity Comment and Pics |
Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 ng/ml. |
Bioassay Procedure |
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG |
Concentration |
0.25mg/ml (determined by Absorbance at 280nm) |
Description |
IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation.. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Domain |
21-133aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
Baculovirus |
Formulation |
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Category |
Immunology |
General References |
Shen HH., et al, (2003) J. Immunol. 170 (6): 3296–3305.; ; Sanderson CJ., et al, (1992) Blood. 79 (12): 3101–3109. |
Molecular Weight |
13.9kDa (119aa) |
Predicted nterm |
Met 21 |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.