Item no. |
NKM-ATGP4010-50ug |
Manufacturer |
NKMAX
|
Amount |
50ug |
Category |
|
Type |
Proteins Recombinant |
Format |
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
hIgG-His-Tag |
Purity |
> 90% by SDS - PAGE |
NCBI |
NP_066978 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
CDSIGN, CLEC4L, CD209 antigen isoform 1, CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1, CD_antigen, CD209 |
Available |
|
Antigen Species |
Human |
Bioassay Procedure |
VSKVPSS ISQEQSRQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTWLKAA VGELPEKSKM QEIYQELTRL KAAVGELPEK SKQQEIYQEL TRLKAAVGEL PEKSKQQEIY QELTRLKAAV GELPEKSKQQ EIYQELTQLK AAVERLCHPC PWEWTFFQGN CYFMSNSQRN WHDSITACKE VGAQLVVIKS AEEQNFLQLQ SSRSNRFTWM GLSDLNQEGT WQWVDGSPLL PSFKQYWNRG EPNNVGEEDC AEFSGNGWND DKCNLAKFWI CKKSAASCSR DEEQFLSPAP ATPNPPPA |
Concentration |
0.25mg/ml (determined by Absorbance at 280nm) |
Description |
DC-SIGN/CD209, also known as Dendritic Cell-specific ICAM-3 Grabbing Non-integrin, is a member of the C-type lectin family. It is Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. DC-SIGN on macrophages recognises and binds with high affinity to high-mannose type N-glycans, a class of pathogen associated molecular patterns PAMPs commonly found on viruses, bacteria and fungi. This binding interaction activates phagocytosis. It has a high affinity for the ICAM3 molecule and binds various microorganisms by recognizing high-mannose-containing glycoproteins on their envelopes and especially functions as receptor for several viruses such as HIV and Hepatitis C. Besides functioning as an adhesion molecule, recent study has also shown that DC-SIGN can initiate innate immunity by modulating toll-like receptors, though the detailed mechanism is not yet known. It together with other C-type lectins is involved in recognition of tumors by dendritic cells. It is also a potential engineering target for dendritic cell based cancer vaccine. Recombinant human DC-SIGN/CD209, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Domain |
60-404aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
Baculovirus |
Gene Category |
Immunology |
General References |
Liu, W. et al, (2004) J. Biol. Chem. 279:18748.; ; Anthony, R.M. et al, (2008) Proc. Natl. Acad. Sci. USA 105:19571. |
Internal Search Terms |
ATGP4010-10ug, ATGP4010-20ug, ATGP4010-50ug, ATGP4010-100ug, ATGP4010-250ug, ATGP4010-500ug, ATGP4010-1mg, ATGP4010-10, ATGP4010-20, ATGP4010-50, ATGP4010-100, ATGP4010-250, ATGP4010-500, ATGP4010-1 |
Molecular Weight |
66.5kDa (587aa) |
Predicted nterm |
Ala |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.