Recombinant mouse sFRP-2 protein

LFLFGQP DFSYKRSNCK PIPANLQLCH GIEYQNMRLP NLLGHETMKE VLEQAGAWIP LVMKQCHPDT KKFLCSLFAP VCLDDLDETI QPCHSLCVQV KDRCAPVMSA FGFPWPDMLE CDRFPQDNDL CIPLASSDHL LPATEEAPKV CEACKTKNED DNDIMETLCK NDFALKIKVK EITYINRDTK IILETKSKTI YKLNGVSERD LKKSVLWLKD SLQCTCEEMN DINAPYLVMG QKQGGELVIT SVKRWQKGQR EFKRISRSIR KLQC

sFRP-2, also known as Secreted apoptosis-related protein 1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. sFRP-2 can exert either positive or negative effects on Wnt signaling. At physiological concentrations, it also inhibits BMP-induced effects. It plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation. It is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity. Recombinant mouse sFRP-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 20% glycerol

Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645-35653; ; He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110-21115; ;

Ala