Item no. |
NKM-ATGP3960-50ug |
Manufacturer |
NKMAX
|
Amount |
50ug |
Category |
|
Type |
Proteins Recombinant |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 95% by SDS - PAGE |
NCBI |
NP_828851.1 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
E2 glycoprotein precursor, Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Severe acute repiratory Syndrome-related Coronavirus, SARS, SRAS-CoV, SARS-CoV1 |
Available |
|
Antigen Species |
SARS-CoV |
Bioactivity Comment Only |
Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# ATGP3963) |
Bioassay Procedure |
RVVPSGD VVRFPNITNL CPFGEVFNAT KFPSVYAWER KKISNCVADY SVLYNSTFFS TFKCYGVSAT KLNDLCFSNV YADSFVVKGD DVRQIAPGQT GVIADYNYKL PDDFMGCVLA WNTRNIDATS TGNYNYKYRY LRHGKLRPFE RDISNVPFSP DGKPCTPPAL NCYWPLNDYG FYTTTGIGYQ PYRVVVLSFE LLNAPATVCG PKL |
Concentration |
0.5 mg/ml (determined by Absorbance at 280nm) |
Description |
Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), and the recently identified novel Coronavirus (SARS-CoV-2) belong to the Coronaviridae family, genus Betacoronavirus, that has been related to important epidemiological outbreaks. SARS-CoV emerged in 2003 as a significant threat to human health. SARS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. It attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit. Recombinant SARS-CoV spike RBD protein fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Domain |
306-515aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
Baculovirus |
Formulation |
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
General References |
Kukla M., et al, (2020) J Clin Med. 9:1420.; Ayouba A., et al, (2020) J Clin Virol. 129:104521.; Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93-116.; Li F, et al, (2005) Science. 309:1864-1868.; Struck AW, et al, (2012) Antiviral Res. 94:288-296. |
Molecular Weight |
24.7kDa (219aa) |
Predicted nterm |
Ala |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.