Item no. |
NKM-ATGP3922-20ug |
Manufacturer |
NKMAX
|
Amount |
20ug |
Category |
|
Type |
Proteins Recombinant |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 85% by SDS-PAGE |
NCBI |
NP_000036 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Arginase-1 liver, Arginase-1, liver, Arginase-1, liver A I, Al, ARG 1, ARG1, Arginase 1, Arginase liver, Arginase type I, Arginase1, Liver type arginase, Type I arginase. |
Available |
|
Antigen Species |
Human |
Bioactivity Comment Only |
Specific activity is > 150, 000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of arginine to urea per minute at pH 10.5 at 37C. |
Bioactivity Comment and Pics |
Specific activity is > 150, 000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37C. |
Bioassay Procedure |
MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PK |
Concentration |
0.5mg/ml (determined by Bradford assay) |
Description |
Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. Recombinant human ARG1, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
1-322aa |
Expression System |
E.coli |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl |
Gene Category |
Metabolism |
General References |
Wu G., et al (1998) The Biochemical journal 336 (Pt 1): 1-17.; ; Iyer RK., et al (2002) Molecular and cellular biology 22 (13): 4491-8.; ; |
Molecular Weight |
35.8 kDa (330aa) confirmed by MALDI-TOF |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.