Item no. |
NKM-ATGP3920-20ug |
Manufacturer |
NKMAX
|
Amount |
20ug |
Category |
|
Type |
Proteins Recombinant |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 95% by SDS-PAGE |
NCBI |
NP_000563 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10, GVHDS, IL10A, TGIF |
Available |
|
Antigen Species |
Human |
Bioactivity Comment Only |
Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range <=5ng/ml. |
Bioactivity Comment and Pics |
Human IL-10 stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range <=5 ng/ml. |
Bioassay Procedure |
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN |
Concentration |
0.5mg/ml (determined by Bradford assay) |
Description |
IL-10, also known as Interleukin-10, is an anti-inflammatory cytokine. It inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. This protein controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. Recombinant human IL-10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Domain |
19-178aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
Baculovirus |
Formulation |
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Category |
Hormones & Cytokines |
General References |
Jog NR., et al. (2018) Front Immunol. 9:2198.; ; Harling K., et al. (2019) Cell Mol Immunol. 16:275-287. |
Molecular Weight |
19.4 kDa (166aa) |
Predicted nterm |
Ser19 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.