Item no. |
NKM-ATGP3841-50ug |
Manufacturer |
NKMAX
|
Amount |
50ug |
Category |
|
Type |
Proteins Recombinant |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 95% by SDS-PAGE |
NCBI |
NP_060015 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Hydroxyacid oxidase 1, GOX (glycolate oxidase), GOX1, HAOX1 |
Available |
|
Antigen Species |
Human |
Bioactivity Comment Only |
Specific activity is > 3, 000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C. |
Bioactivity Comment and Pics |
Specific activity is > 3, 000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C. |
Bioassay Procedure |
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI |
Concentration |
1mg/ml (determined by Bradford assay) |
Description |
HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
Domain |
1-370aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
E.coli |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl |
Gene Category |
Metabolism |
General References |
Pennati A., et al. (2009) J. Biol. Chem. 284:31214. |
Molecular Weight |
45.0 kDa (406aa) confirmed by MALDI-TOF |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.