Item no. |
NKM-ATGP3251-100ug |
Manufacturer |
NKMAX
|
Amount |
100ug |
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Conjugate/Tag |
Non-Tagged |
Purity |
> 90% by SDS - PAGE |
Sequence |
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE |
NCBI |
NP_001018149 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B |
Available |
|
Activity |
Specific activity is > 1, 800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH. |
Antigen Species |
Human |
Applications info |
Enzyme Activity, SDS-PAGE |
Biological activity |
Specific activity is > 1, 800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH. |
Concentration |
1 mg/ml (determined by Bradford assay) |
Description |
NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
1-152aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol |
Gene Category |
Metabolism, Signal Transduction, Cancer |
General References |
Munkonge FM., et al. (2009) J Biol Chem. 284(39):26978-87. Treharne KJ., et al. (2009) FEBS Lett. 583(17):2789-92. |
Molecular Weight |
17.2 kDa (152aa), confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.