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Recombinant e.coli ung protein

Recombinant e.coli ung protein

Item no.	NKM-ATGP2365-10ug
Manufacturer	NKMAX
Amount	10ug
Category	Peptides & Proteins Recombinant Proteins SDS-PAGE
Type	Proteins Recombinant
Applications	SDS-PAGE
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 90% by SDS-PAGE
NCBI	NP_417075
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	uracil-DNA-glycosylase, ECK2578, JW2564
Available
Antigen Species	E.coli
Bioassay Procedure	MGSSHHHHHH SSGLVPRGSH MGSMANELTW HDVLAEEKQQ PYFLNTLQTV ASERQSGVTI YPPQKDVFNA FRFTELGDVK VVILGQDPYH GPGQAHGLAF SVRPGIAIPP SLLNMYKELE NTIPGFTRPN HGYLESWARQ GVLLLNTVLT VRAGQAHSHA SLGWETFTDK VISLINQHRE GVVFLLWGSH AQKKGAIIDK QRHHVLKAPH PSPLSAHRGF FGCNHFVLAN QWLEQRGETP IDWMPVLPAE SE
Concentration	0.5mg/ml (determined by Bradford assay)
Description	ung, also known as uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. uracil bases occur from cytosine deamination or misincorporation of duMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Recombinant E. coli ung protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Domain	1-229aa
Expression System	E.coli
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Gene Category	Enzymes & Proteases
General References	Lindahl T. et al. (1977) J Biol Chem. 252 : 3286-3294.; ; Pearl, L. H. et al. (2000) Mutation research 460 : 165-181.; ;
Molecular Weight	28.1 kDa (252aa) confirmed by MALDI-TOF

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10ug

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Delivery expected until 10/4/2024

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