Item no. |
NKM-ATGP2333-0.5mg |
Manufacturer |
NKMAX
|
Amount |
0.5mg |
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 85% by SDS - PAGE |
Sequence |
MGSSHHHHHH SSGLVPRGSH MGSDSEEDEE HTIITDTELP PLKLMHSFCA FKADDGPCKA IMKRFFFNIF TRQCEEFIYG GCEGNQNRFE SLEECKKMCT RDNANRIIKT TLQQEKPDFC FLEEDPGICR GYITRYFYNN QTKQCERFKY GGCLGNMNNF ETLEECKNIC EDGPNGFQVD NYGTQLNAVN NSLTPQSTKV PSLFEFHGPS WCLTPADRGL CRANENRFYY NS |
NCBI |
NP_006278 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
tissue factor pathway inhibitor, EPI, LACI, TFI, TFPI1 |
Available |
|
Antigen Species |
Human |
Applications info |
SDS-PAGE |
Concentration |
1 mg/ml (determined by Bradford assay) |
Description |
TFPI is a protease inhibitor that regulates the tissue factor (TF) -dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The protein inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. TFPI is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. Recombinant human TFPI protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
29-304aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol |
Gene Category |
Cardiovascular |
General References |
Prabhakar, P., et al. (2012) Thromb. Res. 130 (4), 687-689 Alioglu, B., et al. (2012) J Clin Res Pediatr Endocrinol 4 (3), 146-150 |
Molecular Weight |
34.3kDa (299aa) |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.