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Recombinant human NSMCE1 protein

Recombinant human NSMCE1 protein

Item no.	NKM-ATGP2231-100ug
Manufacturer	NKMAX
Amount	100ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 95% by SDS - PAGE
Sequence	MGSSHHHHHH SSGLVPRGSH MGSMQGSTRR MGVMTDVHRR FLQLLMTHGV LEEWDVKRLQ THCYKVHDRN ATVDKLEDFI NNINSVLESL YIEIKRGVTE DDGRPIYALV NLATTSISKM ATDFAENELD LFRKALELII DSETGFASST NILNLVDQLK GKKMRKKEAE QVLQKFVQNK WLIEKEGEFT LHGRAILEME QYIRETYPDA VKICNICHSL LIQGQSCETC GI
NCBI	NP_659547
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Non-structural maintenance of chromosomes element 1 homolog, NSE1
Available
Antigen Species	Human
Applications info	SDS-PAGE
Concentration	0.5 mg/ml (determined by Bradford assay)
Description	NSMCE1 is a component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). NSMCE1 has in vitro ubiquitin ligase activity in presence of NDNL2. Recombinant human NSMCE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Domain	1-266aa
Expression System	E.coli
Formulation	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
Gene Category	Epigenomics (Transcription & Translation)
General References	Hudson JJ, Bednarova K, et al. (2011). PLoS One. 6(2):e17270.
Molecular Weight	33 kDa (289aa), confirmed by MALDI-TOF
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ug

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Delivery expected until 8/30/2024

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