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Recombinant e.coli glpE protein

Recombinant e.coli glpE protein

Item no.	NKM-ATGP2144-20ug
Manufacturer	NKMAX
Amount	20ug
Category	Neuroscience Targets for Rodent Neurosciences Peptides & Proteins Recombinant Proteins Cell Biology Cytoskeleton SDS-PAGE small GTPases Rho Subfamily
Type	Proteins Recombinant
Applications	SDS-PAGE
Specific against	other
Conjugate/Tag	His-Tag
Purity	> 95% by SDS-PAGE
NCBI	NP_417883
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Thiosulfate:cyanide sulfurtransferase (rhodanese), ECK3411, JW3388
Available
Antigen Species	E.coli
Bioassay Procedure	MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A
Concentration	0.5mg/ml (determined by Bradford assay)
Description	Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. Recombinant E. coli glpE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Domain	1-108aa
Expression System	E.coli
Formulation	Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Gene Category	Metabolism
General References	Li H., et al. (2011) Biomol NMR Assign. 5(1):97-9; ; Ray WK., et al. (2000) J Bacteriol. 182(8):2277-84.
Molecular Weight	14.5 kDa (131aa) confirmed by MALDI-TOF

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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