Item no. |
NKM-ATGP2056-20ug |
Manufacturer |
NKMAX
|
Amount |
20ug |
Category |
|
Type |
Proteins |
Applications |
SDS-PAGE |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 90% by SDS-PAGE |
NCBI |
NP_417436 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Adenine DNA glycosylase, ECK2956, JW2928, mica, mutB |
Available |
|
Antigen Species |
E.coli |
Bioassay Procedure |
MGSSHHHHHH SSGLVPRGSH MGSMQASQFS AQVLDWYDKY GRKTLPWQID KTPYKVWLSE VMLQQTQVAT VIPYFERFMA RFPTVTDLAN APLDEVLHLW TGLGYYARAR NLHKAAQQVA TLHGGKFPET FEEVAALPGV GRSTAGAILS LSLGKHFPIL DGNVKRVLAR CYAVSGWPGK KEVENKLWSL SEQVTPAVGV ERFNQAMMDL GAMICTRSKP KCSLCPLQNG CIAAANNSWA LYPGKKPKQT LPERTGYFLL LQHEDEVLLA QRPPSGLWGG LYCFPQFADE ESLRQWLAQR QIAADNLTQL TAFRHTFSHF HLDIVPMWLP VSSFTGCMDE GNALWYNLAQ PPSVGLAAPV ERLLQQLRTG APV |
Concentration |
0.25mg/ml (determined by Bradford assay) |
Description |
Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Recombinant E. coil mutY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
1-350aa |
Expression System |
E.coli |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol |
Gene Category |
Enzymes & Proteases |
General References |
Lee H M., et al. (2004) J Neurochem. 88:394-400; ; Tao H., et al. (2004) Carcinogenesis. 25:1859-1866.; ; |
Molecular Weight |
41.5 kDa (373aa) confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.