Comparison

Recombinant human UbcH5b/UBE2D2 protein

Item no. NKM-ATGP1147-0.5mg
Manufacturer NKMAX
Amount 0.5mg
Category
Type Proteins Recombinant
Specific against other
Conjugate/Tag His-Tag
Purity > 95 % by SDS - PAGE
Sequence MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM
NCBI NP_003330
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias ubiquitin-conjugating enzyme E2 D2, E2(17)KB2, PuBC1, uBC4, uBC4/5, uBCH5B
Available
Antigen Species
Human
Applications info
SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay)
Description
uBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. uBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human uBE2D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Domain
1-147aa
Expression System
E.coli
Formulation
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Gene Category
Ubiquitination
General References
Ciechanover A., et al. (1994) FASEB J. 8(2):182-19.
Orian A., et al. (1995) J Biol Chem. 270(37):21707-14.
Molecular Weight
18.9 kDa (167aa), confirmed by MALDI-TOF
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.5mg
Available: In stock
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