Item no. |
NKM-ATGP1147-0.5mg |
Manufacturer |
NKMAX
|
Amount |
0.5mg |
Category |
|
Type |
Proteins Recombinant |
Specific against |
other |
Conjugate/Tag |
His-Tag |
Purity |
> 95 % by SDS - PAGE |
Sequence |
MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM |
NCBI |
NP_003330 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
ubiquitin-conjugating enzyme E2 D2, E2(17)KB2, PuBC1, uBC4, uBC4/5, uBCH5B |
Available |
|
Antigen Species |
Human |
Applications info |
SDS-PAGE |
Concentration |
0.5 mg/ml (determined by Bradford assay) |
Description |
uBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. uBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human uBE2D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
1-147aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl. |
Gene Category |
Ubiquitination |
General References |
Ciechanover A., et al. (1994) FASEB J. 8(2):182-19. Orian A., et al. (1995) J Biol Chem. 270(37):21707-14. |
Molecular Weight |
18.9 kDa (167aa), confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.